A validated HPLC method was developed to determine doxorubicin concentration in a small volume of rat plasma (60 µl) with convenient fluorescence detection. Sample preparation includes a simple one-step liquid-liquid extraction using minimum amount of organic solvent, with extraction recoveries more than 95%. The analysis was accomplished using PerfectSil C18 column maintained at 35 °C and a mo...

a simple and reproducible hplc method with spectrophotometric detection was developed for determination of rivastigmine in human plasma. liquid-liquid extraction of rivastigmine and donepezil (as internal standard) from plasma samples was performed with 1-butanol/n-hexane (2:98 v/v) in alkaline condition followed by back-extraction into diluted acetic acid. chromatography was carried out using ...

The purpose of this study was to separate and determine Nystatin in water, urine and plasma samples using a method based on sodium dodecyl sulfate (SDS) coated magnetic nanoparticles (nano-magnets Fe3O4) along with spectrophotometry which has been developed for to this end. Due to their excellent adsorption capacity and high chemical stability, nanoparticles can be modified by surfactants. The ...

objective: Phenytoin is an anti-seizure medication used to treat epilepsy, as well as to control arrhythmias (irregular heartbeat) and to treat migraine headaches and nerve pain. It is recommended to determine the amount of this drug in the blood to control the seizure and prevent its toxicity. In the present study, a sensitive and simple procedure based on the dispersive solid phase extraction...

an air–assisted liquid–liquid microextraction method for the extraction and preconcentration of trace amounts of some synthetic phenolic antioxidants in biological fluids followed by their determination by gas chromatography–flame ionization detection has been reported. in this method the target analytes are extracted into a few microliters of carbon tetrachloride (extraction solvent) from an a...

Electromembrane extraction (EME) of the polar zwitterionic drugs, anthracyclines (ANT, doxorubicin, daunorubicin and its metabolite daunorubicinol), from rabbit plasma was investigated. The optimized EME compared to conventional sample pretreatment techniques such as protein precipitation (PP) liquid-liquid (LLE), mainly in terms reliability, recovery matrix effect. In addition, phospholipids p...

A validated HPLC method was developed to determine doxorubicin concentration in a small volume of rat plasma (60 µl) with convenient fluorescence detection. Sample preparation includes a simple one-step liquid-liquid extraction using minimum amount of organic solvent, with extraction recoveries more than 95%. The analysis was accomplished using PerfectSil C18 column maintained at 35 °C and a mo...

A simple and reproducible HPLC method with spectrophotometric detection was developed for determination of rivastigmine in human plasma. Liquid-liquid extraction of rivastigmine and donepezil (as internal standard) from plasma samples was performed with 1-butanol/n-hexane (2:98 v/v) in alkaline condition followed by back-extraction into diluted acetic acid. Chromatography was carried out using ...

N-Arachidonoylethanolamine (AEA, anandamide) was the first endocannabinoid to be identified and has since become associated with the mediation of several physiological functions and disease states. AEA has been isolated from numerous tissues and biofluids, in the low nanomolar range, using lipid extraction techniques with organic solvents. These techniques require the drying down of relatively ...

The relevance of local removal and release of norepinephrine (NE) for antecubital venous plasma NE concentration was studied in 22 healthy subjects. Arterial and venous plasma NE and forearm blood flow were measured during intra-arterial infusion of two doses of NE, intra-arterial NE infusion with two doses of sodium nitroprusside, intravenous infusion of NE with intra-arterial infusion of four...