A newly modified semimicro plaque reduction neutralization test (PRNT) in BHK cells was compared with a standard PRNT in bottles with LLC-MK2 monolayers and with an LLC-MK2 PRNT adapted to semimicro methods. The BHK semimicro PRNT compared favorably in terms of sensitivity in detecting dengue antibody (96%), specificity at a screening dilution (95%), and ability to detect seroconversion to deng...

Rift Valley fever virus (RVFV) is an important human and animal pathogen in Africa and has been responsible for infections in travelers. Because of the aerosol infectivity and risk of dissemination of the virus, a need exists for simple, safe, serological tests for diagnosis. An enzyme-linked immunosorbent assay (ELISA) was developed to detect RVFV-specific immunoglobulins (immunoglobulin G [Ig...

West Nile (WN) virus was introduced into the United States in 1999, when the first human cases of WN fever and encephalitis appeared in New York City. From there, the virus has spread throughout North America, in some areas cocirculating with the related flavivirus St. Louis encephalitis (SLE) virus. Public health laboratories currently use an immunoglobulin M (IgM) antibody capture enzyme-link...

BACKGROUND Accurate determination of neutralization antibody titers supports epidemiological studies of dengue virus transmission and vaccine trials. Neutralization titers measured using the plaque reduction neutralization test (PRNT) are believed to provide a key measure of immunity to dengue viruses, however, the assay's variability is poorly understood, making it difficult to interpret the s...

Jahiel, R. I. (Cornell University Medical College, New York, N.Y.), and E. D. Kilbourne. Reduction in plaque size and reduction in plaque number as differing indices of influenza virus-antibody reactions. J. Bacteriol. 92:1521-1534. 1966.-The serological reactivity of an antigenically hybrid influenza virus recombinant (X-7) was studied in a heteroploid cell plaquing system in which antisera to...

Neutralization of vaccinia virus with immune rabbit serum occurred optimally when incubated at 37 C for 16 to 24 hr in the plaque neutralization test employing the MA-104 embryonic rhesus monkey kidney cell line.

of the Joint International Tropical Medicine Meeting (JITMM). Bangkok, Thailand. 29 November-1 December 2004:106. EVALUATION OF A SINGLE-DILUTION PLAQUE REDUCTION NEUTRALIZATION TEST (PRNT) AS A DIAGNOSTIC TOOL FOR DETECTING INTERCURRENT DENGUE VIRUS (DENV) INFECTIONS Thomas SJ, Nisalak A, Halstead S, Endy T, Lutthiwongsakorn N, Poolpanichupatam Y, Sawatwong P, Changnak S and Mammen MP Jr Studi...