A simple and efficient plantlet regeneration protocol via direct organogenesis was established for camphor tree (Cinnamomum camphora L.). Stem segments with one node (SN explants) from embryo-cultured seedlings (EC seedlings) were used as explants. Murashige and Skoog (MS) medium supplemented with 0.5 mg/L 2, 4-dichlorophenoxyacetic acid and 2.0 mg/L 6-benzyladenine was used to induce cotyledon...

Micropropagation protocol of Pimpinella candolleana an important ethno medicinal herb has been developed for the first time. High frequency plantlet regeneration was achieved from shoot tip explants of P. candolleana. Effect of growth regulators in different concentrations and combination of BAP, KN, NAA, IAA and IBA on in vitro morphogenesis was tried. The highest regeneration response was obs...

Rhizome explants were used to develop a protocol for in vitro plantlet regeneration of Podophyllum hexandrum, a critically endangered medicinal plant, through direct organogenesis. Highest rate of multiple shoot formation was noted in MS medium supplemented with 11.42 μM IAA within three months. A synergistic effect of 2.68 μM NAA and 11.1 μM BAP was second best. The 1⁄2 MS liquid medium with 1...