The peptide from peptones responsible for enhanced pigment production by Streptococcus agalactiae in culture media has been isolated from a peptic digest of human albumin and has been identified as Ile-Ala-Arg-Arg-His-Pro-Tyr-Phe. The related heptapeptide lacking the N-terminal Ile also had pigment-enhancing activity. A sequence similarity search showed that these sequences are present only in ...

A biphasic culture medium suitable for cultivation and sporulation of Clostridium perfringens, C. botulinum, and C. sporogenes was devised. The medium designed for use in a disposable, compartmented, plastic film container contained peptones, yeast extract, minerals, an anion exchange resin, and glucose in 4% agar as the solid phase and (NH(4))(2)SO(4) and 0.1% agar as the liquid phase. With th...

In previous reports we have described the toxicity of certain peptones and amino acids, particularly cystine, for brucellae (Schuhardt, Rode, Foster, and Oglesby, 1949; Schuhardt, Rode, and Oglesby, 1949). Subsequently, we found and reported that cystine per se is not toxic for these organims, and proved that the observed toxicity of autoclaved cystine is due to a heat-induced breakdown product...

A habitat-simulating medium was developed for the enumeration and isolation of bacteria from a swine waste digester. A roll tube medium with growth factors for strict anaerobes from previously studied anaerobic ecosystems was used to evaluate the effects of deletion, addition, or level of digester fluid, digester fluid treated with acid or base, rumen fluid, fecal extract, anaerobic pit extract...

Glucagon like peptide-1 is an insulinotropic hormone released from intestinal L-cells in response to food ingestion. Here, we investigated mechanisms underlying the sensing of peptones by primary small intestinal L-cells. Meat, casein and vegetable-derived peptones (5 mg/ml), the L-amino acids Phe, Trp, Gln and Ala (20 mM each), and the dipeptide glycine-sarcosine (20 mM) stimulated GLP-1 secre...

That proteins and their constituents combine with heavy met’als to form “complexes” has been known for some time. Among these copper has received the most attention and considerable work has been done on the copper complexes of amino-acids; t’hey have been isolated,2 analyzed and studied by chemical and physical methods. On the other hand the complexes of peptides and peptones have received onl...

The canning industry plays a crucial role in the sector of marine foods, but produces several organic wastes and effluents with reduced economic value that must be upgraded to comply circular bioeconomy principles. Initially, we have searched best experimental conditions for hydrolysis upper lower halves from yellowfin tuna ( Thunnus albacares ) heads by four commercial proteases (alcalase, pap...