Background A glycolipopeptide biosurfactant produced by Pseudomonas aeruginosa strain IKW1 reduced the surface tension of fermentation broth from 71.31 to 24.62 dynes/cm at a critical micelle concentration of 20.80 mg/L. The compound proved suitable for applications in emulsion stabilization in food, as well as in cosmetic and pharmaceutical formulations. Method In the present study, Plackett...

background: hydrocarbons degradation is principally achieved by microorganisms in natural environments. the extent of hydrocarbons biodegradation is mainly conditioned by environmental factors and its success depends on the optimal condition for the crude oil degrading isolates. objectives: the aims of the current study was to isolate and identify crude oil degrading bacterium from surface sedi...

In the present study, the oleaginous fungus Mortierella alpina CBS 754.68 was screened for arachidonic acidproduction using inexpensive agricultural by-products as substrate. Four oilcakes were analysed to choose the best substrate among them. Sunflower oilcake was the most effective substrate for ARA production followed by soybean, colza and olive oilcakes. In the next step, seven variables in...

Bio-succinic acid production using microorganisms has been interesting as an environmentally friendly process. Palm oil mill effluent (POME) was considered a cheap substrate to lower the cost of production. It revealed that 2-fold diluted POME produced more succinic than undiluted and 5-fold POME. In addition, effects various neutralizing agents on utilized manage pH CO2 supply indicated utiliz...

Green chemistry - also called sustainable chemistry - as the cost-effective and environmentally friendly techniques have been gaining more attention recent years. Here, we introduced a fast, non-toxic and sustainable method to synthesize Ag nanoparticles. Various parameters are involved in the bio-synthesis of Ag nanoparticles/multi-walled carbon nanotubes (Ag/MWf-CNT) composites, including...

Nisin is a bacteriocin approved in more than 50 countries as a safe natural food preservative. Response surface methodology (RSM) combined with artificial neural network-genetic algorithm (ANN-GA) was employed to optimize the fermentation medium for nisin production. Plackett-Burman design (PBD) was used for identifying the significant components in the fermentation medium. After that, the path...

Background: Hydrocarbons degradation is principally achieved by microorganisms in natural environments. The extent of hydrocarbons biodegradation is mainly conditioned by environmental factors and its success depends on the optimal condition for the crude oil degrading isolates. Objectives: The aims of the current study was to isolate and identify crude oil degrading bacterium from surface sed...

The objective of the study was to optimize the nutrition sources in a culture medium for the production of xylanase from Penicillium sp.WX-Z1 using Plackett-Burman design and Box-Behnken design. The Plackett-Burman multifactorial design was first employed to screen the important nutrient sources in the medium for xylanase production by Penicillium sp.WX-Z1 and subsequent use of the response sur...

Alkaline protease producing microorganism Vibrio metschnikovii NG155, isolated from the soil samples of leather industry, having potential application in dehairing of animal skin was used in this study. To enhance the production of protease by this strain, various physical and nutritional factors were optimized using statistical methods like Plackett Burman and Response Surface Methodology. In ...

In this study, the optimization of medium components used for production lipase enzyme from Cryptococcus albidus D24 was performed using Plackett-Burman statistical design method (PBD), and most important nutrients affecting strain were determined as first step. According to PBD, highest activity (19.34 U/ml/min) obtained with including Tween 80 (X2) 2.5% (v/v), (g/L) Peptone (X4) 8.0, Yeast Ex...