نتایج جستجو برای: pkm2

تعداد نتایج: 844  

Journal: :Asian Pacific journal of cancer prevention : APJCP 2015
Nian Luo Lv-Cui Zhao Qing-Qiang Shi Zi-Qiang Feng Di-Long Chen Jing Li

BACKGROUND Diallyl disulfide (DADS) may exert potent anticancer action both in vitro and in vivo. Although its effects on cancer are significant, the underlying mechanisms remain unknown. In this study, we sought to elucidate possible links between DADS and pyruvate kinase (PKM2). MATERIALS AND METHODS KG1α, a leukemia cell line highly expressing PKM2 was used with a cell counting kit (CCK)-8...

Journal: :Molecular cell 2013
Lei Lv Yan-Ping Xu Di Zhao Fu-Long Li Wei Wang Naoya Sasaki Ying Jiang Xin Zhou Ting-Ting Li Kun-Liang Guan Qun-Ying Lei Yue Xiong

Alternative splicing of the PKM2 gene produces two isoforms, M1 and M2, which are preferentially expressed in adult and embryonic tissues, respectively. The M2 isoform is reexpressed in human cancer and has nonmetabolic functions in the nucleus as a protein kinase. Here, we report that PKM2 is acetylated by p300 acetyltransferase at K433, which is unique to PKM2 and directly contacts its allost...

2015
Alba Ginés Sara Bystrup Vicenç Ruiz de Porras Cristina Guardia Eva Musulén Anna Martínez-Cardús José Luis Manzano Laura Layos Albert Abad Eva Martínez-Balibrea

Chemoresistance is the main cause of treatment failure in advanced colorectal cancer (CRC). However, molecular mechanisms underlying this phenomenon remain to be elucidated. In a previous work we identified low levels of PKM2 as a putative oxaliplatin-resistance marker in HT29 CRC cell lines and also in patients. In order to assess how PKM2 influences oxaliplatin response in CRC cells, we silen...

Journal: :Molecular medicine reports 2015
Beibei Chu Jiang Wang Yueying Wang Guoyu Yang

The M2 isoform of pyruvate kinase (PKM2), which has been identified as the predominant cause of the Warburg effect in cancer cells, is essential in tumor metabolism and growth. However, the role of PKM2 in autophagy remains to be elucidated. The present study investigated the effect of PKM2 knockdown on autophagy and apoptotic cell death in human A549 alveolar adenocarcinoma cells. Two short ha...

2017
Xing Wang Fenglin Zhang Xue-Ru Wu

Chemoresistance to cisplatin is a principal cause of treatment failure and mortality of advanced bladder cancer (BC). The underlying mechanisms remain unclear, which hinders the development of preventive strategies. Recent data indicate that pyruvate kinase M2 (PKM2), a glycolytic enzyme for Warburg effect, is strongly upregulated in BC. This study explores the role of PKM2 in chemoresistance a...

2017
Bo Han Xiangqi Meng Hui Chen Lingchao Chen Xing Liu Hongjun Wang Daming Liu Fei Gao Lin Lin Jianguang Ming Bo Sun Shi Yin Ruijia Wang Pengfei Wu Jinquan Cai Chuanlu Jiang

Glioblastoma is the most malignant type of brain tumor, and its high invasiveness and multiplication severely shortens patients' overall survival. The embryonic pyruvate kinase M2 (PKM2) isoform is highly expressed in human cancer. We used computational target gene prediction, in vitro cell culture, immunoblotting, quantitative real-time PCR, ATP measurements, luciferase reporter assays, wound-...

2017
Wei Wang Qianting He Jingjing Sun Zhonghua Liu Luodan Zhao Zhiyuan Lu Xiaofeng Zhou Anxun Wang

Pyruvate kinase M2 (PKM2) has been verified to correlate with the prognosis of many types of cancer. However, its role in the development and metastasis of tongue squamous cell carcinoma (TSCC) remains unclear. The immunohistochemistry (IHC) results confirmed that PKM2 is overexpressed in patients with TSCC. PKM2 up-regulation was related to lymph node metastasis and associated with reduced ove...

Journal: :American journal of cancer research 2016
Ming-Chuan Hsu Wen-Chun Hung Hirohito Yamaguchi Seung-Oe Lim Hsin-Wei Liao Chia-Hua Tsai Mien-Chie Hung

Pyruvate kinase is a key enzyme in the glycolytic pathway that converts phosphoenolpyruvate to pyruvate, and the M2 isoform of pyruvate kinase (PKM2) is associated with cancer. PKM2 has been reported to function independently of its pyruvate kinase activity, which is crucial for cancer cell proliferation. Moreover, there is growing evidence indicating that dimeric PKM2 is released from tumor ce...

2015
Akiko Suzuki Sachin Puri Bharat H Joshi Pamela Leland Bernard A Fox Raj K Puri

Non-small cell lung cancer (NSCLC) is the most common cause of cancer related mortality worldwide and in need of new treatment options. Previously, we have shown that 97 genes are overexpressed in 9 primary NSCLC cell lines by DNA microarray technology. Pyruvate kinase M2 (PKM2), an allosteric isoform of pyruvate kinase and metabolic enzyme necessary for aerobic glycolysis and cell proliferatio...

Journal: :Molecular cell 2015
Aaron M Hosios Brian P Fiske Dan Y Gui Matthew G Vander Heiden

The role of pyruvate kinase M2 (PKM2) in cell proliferation is controversial. A unique function of PKM2 proposed to be important for the proliferation of some cancer cells involves the direct activity of this enzyme as a protein kinase; however, a detailed biochemical characterization of this activity is lacking. Using [(32)P]-phosphoenolpyruvate (PEP) we examine the direct substrates of PKM2 u...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید