Multilamellar stacking seen in negatively stained lipid vesicle-tubulin mixtures has been attributed to lipid-protein interactions (Caron, J. M., and R. D. Berlin, 1979, J. Cell Biol. 81:665-671). We show that this stacking is produced by the phosphotungstic acid used for staining, independent of the presence of tubulin in the sample. The morphology of negatively stained single bilayer vesicles...

An alkaline phosphotungstate procedure was developed in which the phosphotungstic acid reagent is used both as protein-precipitating and oxidizing reagent. The procedure requires only a 0.2-mi sample and fewer reagents and manipulations than similar procedures, but yields results equivalent to those obtained by Henry’s alkaline phosphotungstate procedure. Recovery, precision, and linearity were...

Grafting of 3-aminopropyltriethoxysilane (APTS) on graphene oxide (GO) nanosheets followed by reaction with phosphotungstic acid (H3PW12O40, denoted as H3PW) gave a new functionalized GO which was characterized using FT-IR, FESEM, EDX, EDX elemental mapping and ICP-OES techniques. The catalytic activity of this nanomaterial containing phosphotungstic counter-anion H2PW12O40¯ (H2PW) which was de...

THE seromucoid* fraction of the plasma proteins comprises a group of glycoproteins-i.e., protein-carbohydrate complexes-characterized by their solubility in certain protein precipitants (perchloric, sulphosalicylic and trichloracetic acids) and by being not readily precipitated by heat. This fraction, which normally contains about io% of the total proteit-bound carbohydrate of the plasma and ac...

A simple and efficient one-pot, three-component Strecker reaction of protected amino acids, aromatic aldehydes, and trimethylsilyl cyanide has been developed for the synthesis of chiral α-amino nitriles. The reaction was carried out in the presence of catalytic amount of phosphotungstic acid (H3[P(W3O10)4]) as an environmentally friendly cata...

A procedure is described whereby highly purified fractions of plasma membrane and tonoplast were isolated from hypocotyls of dark-grown soybean (Glycine max L. var Wayne) by the technique of preparative free-flow electrophoresis. Fractions migrating the slowest toward the anode were enriched in thick (10 nanometers) membranes identified as plasma membranes based on ability to bind N-1-naphthylp...