A method is reported for the phosphoryl bond cleavage of O-alkyl phosphinates, phosphinothioates and certain phosphonamidates to furnish the corresponding P(III) borane adducts. The two-step procedure relies upon initial activation of the phosphoryl bond with an alkyl triflate, followed by reduction of the resulting intermediate using lithium borohydride.

Applications of isotopic techniques have contributed considerably to the understanding of kinase mechanisms. Studies with 018 (l-3) have shown that various kinase reactions proceed by transfer of a phosphoryl group without exchange of the phosphoryl oxygens with water or substrates’ Evidence that the kinase reactions do not involve formation of a phosphoryl enzyme intermediate is given by the i...

The Bacillus subtilis sacU locus consists of two genes, degS and degU, which positively regulate the synthesis of several extracellular enzymes including the neutral and alkaline proteases. Both the DegS and DegU proteins have been purified from overproducing Escherichia coli strains harboring degS or degU gene-carrying plasmids, and the following results were obtained. DegS was autophosphoryla...

A protein designated phosphor!// Iransferasr has bee11 isolated from beef heart mitochondria and has been found to increase the ability of suhnlitocllorldrial particles, oxidizing either NASH or snecinatc, to synthesize ,4TP from ADI and inorganic orthophosphate. Increases in the value of P:O ratios between 0.45 and 1 have been observed wit,h srlccinate as snbstrat e. Phosphoryl trnnsferase was...

Applications of isotopic techniques have contributed considerably to the understanding of kinase mechanisms. Studies with 018 (l-3) have shown that various kinase reactions proceed by transfer of a phosphoryl group without exchange of the phosphoryl oxygens with water or substrates’ Evidence that the kinase reactions do not involve formation of a phosphoryl enzyme intermediate is given by the i...

The Cori ester α-d-glucose 1-phosphate (αGlc 1-P) is a high-energy intermediate of cellular carbohydrate metabolism. Its glycosidic phosphomonoester moiety primes αGlc 1-P for flexible exploitation in glucosyl and phosphoryl transfer reactions. Two structurally and mechanistically distinct sugar-phosphate phosphatases from Escherichia coli were characterized in this study for utilization of αGl...

The P atom in the title compound, C(16)H(17)Cl(3)N(3)O(2)P, is bonded in a distorted tetra-hedral geometry with the phosphoryl and carbonyl groups anti with respect to one another. In the crystal, mol-ecules are linked through (N-H)(2)⋯O(=P) and N-H⋯O(=C) hydrogen bonds into chains along [001]. The phosphoryl O atom acts as a double hydrogen-bond acceptor.

In both independent mol-ecules of the title compound, [Nd(C9H11NO4P)3(C12H8N2)], the Nd(III) atom is coordinated by six O atoms belonging to three phosphoryl ligands and two N atoms of 1,10-phenanthroline in a dodeca-hedral geometry. In the phosphoryl ligands, the benzene rings are twisted with respect to the planes of the sp(2)-hybridized C atoms of the chelate rings by 12.1 (1)-24.7 (1)°.