Pyridine is a prototypical inducer of cytochrome P450 (CYP) 2E1, an enzyme associated with cellular oxidative stress and membrane damage. To better understand the effect of this treatment on cellular lipids, the influence of pyridine exposure (100 mg/kg/day i.p. for 5 days) on fatty acids, fatty esters, and fatty alcohol ethers in brain, heart, liver, and adipose tissue from male Swiss Webster ...

INTRODUCTION In the course of in vitro systems search for the toxicity screening of therapeutic drugs (TDs), different cellular models have been applied to examine their adverse effects in isolated organs. This article describes a simple method to determine effects of TDs at the cell membrane level. The cell membrane is an assembly of proteins and lipids that separate inside from outside, prote...

The lipid composition of the human lens is distinct from most other tissues in that it is high in dihydrosphingomyelin and the most abundant glycerophospholipids in the lens are unusual 1-O-alkyl-ether linked phosphatidylethanolamines and phosphatidylserines. In this study, desorption electrospray ionization (DESI) mass spectrometry-imaging was used to determine the distribution of these lipids...