A possible role for an acidic subcellular compartment in biosynthesis of lung surfactant phospholipids was evaluated with granular pneumocytes in primary culture. Incubation with chloroquine (100mum) was used to perturb this compartment. With control cells, incorporation of [9,10-(3)H]palmitic acid into total lipids and into total phosphatidylcholines increased linearly with time up to 4h. Tota...

Biosynthesis of phosphatidylcholine in young seedlings of Raphanus sativus is supplied with choline from degradation of the seed constituent sinapine (sinapoylcholine). This conclusion has been deduced from the following results: (1) Raphanus sativus seedlings accumulate approx. 70 nmol phosphatidylcholine which may be relevant for the consumption of choline, liberated during hydrolysis of appr...

Abstract Oil in water emulsions are commonly stabilized by emulsifying constituents like proteins and/or low molecular weight emulsifiers. The can compete or coexist at the interface. Interfacial properties thus depend on structure of and oil phase resulting interactions. present study systematically analyzed impact fatty acid saturation triacylglycerides phosphatidylcholine interfacial a β-lac...

A hydrogen-bonding interaction between phenobarbital or pentobarbital with phosphatidylcholine in chloroform is indicated by the effects of added phosphatidylcholine on the infrared and proton magnetic resonance spectra of these barbiturates. The nitrogenbound proton of the barbiturate and the orthophosphate moiety of the phosphatidylcholine molecule appear to be involved. The more pronounced e...

In barley, glycine betaine is a metabolic end product accumulated by wilted leaves; betaine accumulation involves acceleration of de novo synthesis from serine, via ethanolamine, N-methylethanolamines, choline, and betaine aldehyde (Hanson, Scott 1980 Plant Physiol 66: 342-348). Because in animals and microorganisms the N-methylation of ethanolamine involves phosphatide intermediates, and becau...

Biosynthetic pathways of phosphatidylcholine and triglyceride were studied in proliferating hepatic endoplasmic reticulum of rats pretreated with phenobarbital. Phosphatidylcholine accounted for the major increment in membrane phospholipid. In vitro measurements of hepatic microsomal enzymes which catalyze phosphatidylcholine biosynthesis revealed a significant increase in specific activity of ...

A novel, efficient and simple approach for soy phosphatidylcholine analysis according to its fatty acid composition was studied with reverse-phase high-performance liquid chromatography. The reverse-phase high-performance liquid chromatography analysis was performed isocratically using UV detector and simple mobile phase solvents consisting of isopropyl alcohol, methanol, and deionized water in...