BACKGROUND Thiamine (vitamin B(1)) deficiency is associated with severe diseases such as beriberi and Wernicke encephalopathy. Although most Americans have sufficient dietary intake, thiamine deficiency is observed in the alcohol-dependent and elderly populations. Measurement of thiamine concentration in whole blood provides an assessment of vitamin B(1) status in at-risk individuals. METHOD ...

We have measured CO2 fluxes across phosphate solutions at different carbonic anhydrase concentrations, bicarbonate concentration gradients, phosphate concentrations, and mobilities. Temperature was 22-25 degrees C, the pH of the phosphate solutions was 7.0-7.3. We found that under physiological conditions of pH and pCO2 a facilitated diffusion of CO2 occurs in addition to free diffusion when (a...

In the course of an investigation of the chemical phosphorylation of ribonucleasel (cf. (l)), it became necessary to develop a chromatographic procedure for the fractionation of the phosphorylated protein. The method of Hirs et al. (2), employing the weak cation exchanger Amberlite XE-64, is an extremely valuable method for the chromatographic purification of pancreatic ribonuclease, but the us...

A new sensitive, simple, rapid and precise reversed phase high performance liquid chromatographic (HPLC) and a spectrophotometric method has been developed for estimation of tolterodine in tablet dosage form. Chromatography was performed by using the mobile phase, Potassium Phosphate pH 4.5 and acetonitrile mixed by a low pressure gradient program. Hypersil BDS C18 column was used. The waveleng...

Hydroxyapatite column chromatography can be used to purify filamentous bacteriophage--the phage most commonly used for phage display. Virions that have been partially purified from culture supernatant by two cycles of precipitation in 2% polyethylene glycol are adsorbed onto the matrix at a density of at least 7.6 x 10(13) virions (about 3 mg) per milliliter of packed bed volume in phosphate-bu...