Parathyroid glands from laying hens were also  xed in percent glutaraldehyde in phosphate buffer (0.1 M, 7.3 pH) for araldite embedding and used for electron microscopy. For routine histological observations, sections of 5 to 6 um thickness were cut and stained by, Mayer’s haemalum and eosin-phloxine, Masson’s trichrome, Crossman’s modi cation of Mallory’s triple stain, van Gieson’s method fo...

spontaneous abortion is the most common complication of pregnancy. several lines of evidence indicate that immunologic effector cells may play a role in the pathogenesis of idiopathic repetitive abortions. leukocytes form a substantial proportion of stromal cells in decidua and the endometrial granulated lymphocytes (egl) is the predominant decidual leukocyte population in the first trimester o...

The proliferative activity in endometrial stromal granulocytes was studied using two approaches. Firstly, mitotic activity was studied in paraffin wax embedded sections of normal non-pregnant endometrium and early pregnancy decidua stained with phloxine-tartrazine. Secondly, the monoclonal antibody Ki67 was applied to cryostat sections of similar tissues. Endometrial stromal granulocytes were i...

THE function of the pineal organ in man and other mammals is still an enigma, although gradually accumulating evidence suggests a relation to antigonadotrophic activity [3]. The state of uncertainty regarding the functional significance of the pineal organ is at least partly due to the lack of evidence clearly demonstrating secretory activity within the pineal. Previously described pineal paren...

A novel approach for on-line monitoring of drug metabolism in continuously perifused, precision-cut liver slices (PCLS) in a microfluidic system has been developed using high-performance liquid chromatography with UV detection (HPLC-UV). In this approach, PCLS are incubated in a microfluidic device made of poly(dimethylsiloxane) (PDMS) by continuous, single-pass perifusion with fresh medium. Tw...