This study was carried out to investigate the apparent digestibility coefficients (ADCs) value of protein, amino acid and energy and body amino acid composition of rainbow trout fed solvent extracted soybean meal (SBM) and full-fat soybean meal (FFS) partly replacing fish meal (FM) in diets. Five iso nitrogenous (average 50.36% crude protein) and energetic (4294 kcal/kg total energy) ...

the adsorption of a number of amino acids on a defected single-walled carbon nanotube (swcnt) isinvestigated by using the density-functional theory (dft) calculations. the adsorption energies andequilibrium distances are calculated for various configurations such as amino acid attaching to defectsites heptagon, pentagon and hexagon in defective tube and also for several molecular orientationswi...

Throughout, the normal pathway of the metabolism of most amino acids there is a constant loss or int’erchange of amino groups. In the process of deamination keto acids, rather than hydroxy acids, appear to be the chief products (1, 2). In this manner a va.riet)y of keto acids are continuously being generated in the animal body. The fate of these naturally occurring carbonyl compounds is of part...

To investigate the regulatory interactions of amino acid transport and incorporation, we determined the effects of dipeptides on amino acid uptake by bacteria in an estuary and a freshwater lake. Dipeptides noncompetitively inhibited net transport and incorporation of amino acids into macromolecules but had no effect on the ratio of respiration to incorporation. Nearly maximum inhibition occurr...

In this study a perfusion technique has been used to investigate in vivo jejunal absorption and transmural potential difference evoked by the neutral amino acids phenylalanine (56 or 20 mmol/l) and glycine (20 mmol/l), the dibasic amino acid lysine (56 or 5 mmol/l), and a dipeptide glycyl-l-phenylalanine (20 mmol/l) in 11 children with pancreatic insufficiency due to cystic fibrosis and in thre...

Membrane transporters are considered essential for placental amino acid transfer, but the contribution of other factors, such as blood flow and metabolism, is poorly defined. In this study we combine experimental and modeling approaches to understand the determinants of [(14)C]phenylalanine transfer across the isolated perfused human placenta. Transfer of [(14)C]phenylalanine across the isolate...

The l-amino acid ligase RizA from B. subtilis selectively synthesizes dipeptides containing an N-terminal arginine. Many arginyl have salt-taste enhancing properties while Arg-Phe has been found to antihypertensive effect. A total of 21 variants were created by site-directed mutagenesis eight amino acids in the substrate binding pocket. recombinantly produced E. coli and purified affinity chrom...

The transamination by pig mitochondrial and supernatant aspartate transaminase of aromatic amino acids was studied in order to determine if an analogy exists between these enzymes and a previously reported brain phenylalanine transaminase (GEORGE, H., AND GABAY, S. (1968) Biochim. Biophys. Ada, 167, 555). Supernatant and mitochondrial aspartate transaminase from pig heart can utilize aromatic a...

Unnatural amino acid mutagenesis has been used to selectively substitute tyrosine 66 of green fluorescent protein (GFP) with five novel amino acids: p-amino-L-phenylalanine, p-methoxy-L-phenylalanine, p-iodo-L-phenylalanine, p-bromo-L-phenylalanine, and L-3-(2-naphthyl)alanine. The absorbance and emission maxima of the resulting mutant GFPs span the range from 375 to 435 nm and 428 to 498 nm, r...

To determine the relationships among the processes of uptake, intracellular pool formation, and incorporation of amino acids into protein, we measured the uptake of dipeptides and free amino acids by bacterial assemblages in estuarine and coastal waters of the southeast U.S. The dipeptide phenylalanyl-phenylalanine (phe-phe) lowerd V,,,,, of phenylalanine uptake when the turnover rate of phenyl...