The membrane insertase YidC inserts newly synthesized proteins into the plasma membrane. While defects in YidC homologs in animals and plants cause diseases, YidC in bacteria is essential for life. Membrane insertion and assembly of ATP synthase and respiratory complexes is catalyzed by YidC. To investigate how YidC interacts with membrane-inserting proteins, we generated single cysteine mutant...

The effect of oestrogen-containing compounds on platelet factor 3, platelet life span, renal platelet localization and the fibrinolytic activity of forearm venous blood has been studied in eleven women with the renal vascular abnormalities of the loin pain and haematuria syndrome. The results were compared with those obtained in twenty-nine healthy female volunteer subjects. 2. Platelet factor ...

YidC, a 60-kDa integral membrane protein, plays an important role in membrane protein insertion in bacteria. YidC can function together with the SecYEG machinery or operate independently as a membrane protein insertase. In this paper, we describe two new yidC mutants that lead to a cold-sensitive phenotype in bacterial cell growth. Both alleles impart a cold-sensitive phenotype and result from ...

Although salts of the PBr4 and PC14 anions are well-known [1-3], a salt of the corresponding fluoroanion could only recently be prepared by reaction between N(CH3)4F and PF3 using either CH3CN, CHF3 or excess PF3 as a solvent [4], and the novel PF4 anion is stabilized in the form of its tetramethylammonium salt, N(CH3)4PF4. It possesses a pseudo-trigonal bipyramidal structure with two longer (1...

In ciliary and flagellar axonemes, various discrete structures such as inner and outer dynein arms are regularly arranged on the outer doublet microtubules. Little is known about the basis for their regular arrangement. In this study, proteins involved in the attachment of inner-arm dyneins were searched by a microtubule overlay assay on Chlamydomonas mutant axonemes. A 58-kDa protein (p58) was...

A new method, the time of flight-electron-stimulated desorption ion angular distribution method (TOF-ESDIAD), is described. The method permits the mapping of the lateral momentum distribution of adsorbed species on their adsorption sites. Examples of the study of the rotation of chemisorbed PF3 molecules and the frustrated lateral translation of chemisorbed CO molecules, both on single-crystal ...

Membranous microparticles (MP) appearing in the supernatant plasma of stored platelet concentrates (PC) were analyzed by flow cytometry. Two populations of MP were arbitrarily delineated by light scatter as larger or smaller than 0.5 micron fluorescent beads. An estimate of MP concentration was obtained by adding a known amount of fluorescent beads to each sample before analysis of a set number...