The present study describes the isolation of a protein from Escherichia coli possessing kynurenine aminotransferase (KAT) activity and its identification as aspartate aminotransferase (AspAT). KAT catalyses the transamination of kynurenine and 3-hydroxykynurenine to kynurenic acid and xanthurenic acid respectively, and the enzyme activity can be easily detected in E. coli cells. Separation of t...

BACKGROUND AND OBJECTIVES Clostridium perfringens, a Gram-positive obligate anaerobic bacterium, is able to form resistant spores which are widely distributed in the environment. C. perfringens is subdivided into five types A to E based on its four major alpha, beta, epsilon and iota toxins. The aim of the present study was cloning and expression of C. perfringens type D vaccine strain epsilon ...

Enterovirus A71 (EV-A71) là tác nhân chính gây nên biến chứng nguy hiểm của bệnh Tay Chân Miệng, có thể dẫn đến thương tật, và tử vong ở trẻ nhỏ. Hiện nay, vaccine hợp chất điều trị EV-A71 còn nhiều hạn chế, chưa được áp dụng rộng rãi. Vì thế, nghiên cứu tìm giải pháp thay thế hoặc bổ trợ đang tiến hành, trong đó bẫy virus. Với chức năng bắt, cố định virus, ngăn sự xâm nhiễm, bám cấu trúc từ th...

El Huanglongbing (HLB), es una de las enfermedades más devastadoras los cítricos a nivel mundial y representa seria amenaza para la citricultura mexicana. agente causal bacteria incultivable que pertenece al género Candidatus Liberibacter spp. afecta todas especies cítricos, especialmente agrios. Para su detección, se emplean técnicas moleculares como reacción en cadena polimerasa (QT-PCR), ser...

Interleukin (IL)-13 plays an important role in the pathogenesis of asthma. A polymorphic variant of human IL-13 R130Q, results in substitution of an arginine with a glutamine was shown to be associated with asthma in Chinese Han nationality. We examined the functional consequences of this variant in vitro to investigate whether this variant enhanced functional activity compared with wild type I...

β-mannosidase (EC 3.2.1.25, MANB) dissects the non-reducing end of N-linked mannose moieties of glycoproteins in eukaryotic cells. The human β-mannosidase gene was amplified by RTPCR, cloned and sequenced. The DNA sequence was compared with reported human β-mannosidase DNA sequence and sixteen nucleotide differences were found. The deduced amino-acid sequence showed that seven codons coded the ...

Background: Infections due to Pseudomonas aeruginosa are among the leading causes of morbidity and mortality in patients who suffer from impaired immune responses and chronic diseases such as cystic fibrosis. At present, aggressive antibiotic therapy is the only choice for management of P. aeruginosa infections, but emergence of highly resistant strains necessitated the development of novel alt...

Protease 2A (2Apro) of coxsackievirus B3 (CVB3) plays a major role in viral replication. In case of infection, viral proteins are being synthesized from viral mRNA using host biosynthesis machinery. 2Apro of virus, after being synthesized, exhibits two critical functions, cleavage of viral proteins and breaking eukaryotic initiation factor 4G. The enzyme plays an essential role in viral replic...