A sensitive spectrophotometric method for the determination of trace amounts of chromium(VI) is described. Chromium(VI) forms chlorochromate anion in the presence of acid and chloride ion. The formed anion is extracted in toluene as ion-pair with a cationic dye rhodamine 6G. At 535 nlll absorption maximum molar absorptivity is 2x104 I mol-I em-I. The colour is stable for 4 h and the system obey...

A new simple and sensitive kinetic spectrophotometric method for the determination of silymarin in pure form and in pharmaceutical formulations is described. The method is based on the oxidation of the drug with potassium permanganate at pH 7.0 +/- 0.2. The reaction is followed spectrophotometrically by measuring the decrease in the absorbance at 530 nm. The calibration graph is linear in the r...

A method for the determination of selenium by ion chromatography (IC) after oxygen flask combustion has been developed and applied to the determination of selenium in bulk prednisolone. We found elemental selenium [Se(0)], selenite ion [Se(IV)] and selenate ion [Se(VI)] (ca. 5, 75 and 20%) in the absorbing liquid obtained by oxygen flask combustion of Se(0), Se(IV) and Se(VI). Se(VI) was direct...

This is the published version: McDermott, Geoffrey P., Francis, Paul S., Holt, Kayla J., Scott, Kristen L., Martin, Sheree D., Stupka, Nicole, Barnett, Neil W. and Conlan, Xavier A. 2011, Determination of intracellular glutathione and glutathione disulfide using high performance liquid chromatography with acidic potassium permanganate chemiluminescence detection, Analyst, vol. 136, no. 12, pp. ...

is the basis of most of the calcium methods current in biological chemistry, the amount of oxalate in precipitated calcium oxalate being determined by titrimetric measurement of the permanganate utilized. In this paper we present results showing that the oxalate can be determined with equal accuracy by measurement in the Van Slyke-Neil1 (1924) manometric apparatus, and that the gasometric proce...

Acute toxicity study of potassium permanganate was carried out in Swiss albino mice. Potassium permanganate was administered at dose rate of 0.0, 500, 1000, 1500, 2000, 2500, 3000 and 3500mg/kg body weight to groups 1, 2, 3, 4, 5, 6, 7 and 8, ten per group for LD50 determination. The dead animals were posted for gross lesions. A predetermined dose of 160mg/kg of the chemical was administered to...

Nitrite and nitrate are main stable products of nitric oxide, a pivotal cellular signaling molecule, in biological fluids. Therefore, accurate measurement of the two ions is profoundly important. Nitrite is difficult to be determined for a larger number of interferences and unstable in the presence of oxygen. In this paper, a simple, cost-effective and accurate HPLC method for the determination...

Oxidation–reduction titrations and potentiometric methods provide a convenient experimental means to quantitatively determine compositions of unknown mixtures containing oxidizable/reducible species. Over the past decade, numerous titration methods have appeared in this Journal (1–6) and in standard laboratory manuals (7–10) involving common titrants such as potassium dichromate, iodine, potass...

The biological activity of oxybiotin (1, 2) and of an apparently similar substance (3) has been recently described. Prior to these reports the only compound possessing yeast growth activity comparable to that of biotin was desthiobiotin (4), which exerts its biological activity by virtue of the fact that it is converted into biotin by the yeast cell (5, 6). It was of importance to determine whe...

is the basis of most of the calcium methods current in biological chemistry, the amount of oxalate in precipitated calcium oxalate being determined by titrimetric measurement of the permanganate utilized. In this paper we present results showing that the oxalate can be determined with equal accuracy by measurement in the Van Slyke-Neil1 (1924) manometric apparatus, and that the gasometric proce...