The distribution of the neurotensin-related hexapeptide LANTG within the basal ganglia and its projection targets was studied in turtles, pigeons, and hamsters by using immunohistochemical techniques, radioimmunoassay (RIA), gel chromatography, and high performance liquid chromatography (HPLC).

The increasing demand for enantiopure drugs has led to the development of a variety of stereoselective separation technologies. Among them, high performance liquid chromatography (HPLC) is well recognized as a powerful, fast and efficient technique, which has been successfully employed for analysis and preparation of enantiomers of drugs. Nowadays, liquid chromatographic techniques are the focu...

High performance liquid chromatography (HPLC) identifies compounds in a sample and quantifies their concentration. The instrument consists of an injector that introduces μL to mobile phase carries the analytes across column filled with adsorbing substance. adsorb desorb at different rates as they flow down are thereby separated. Detectors record signal proportional concentration analyte phase. ...

High-performance liquid chromatography (HPLC) is an essential tool for the purification and characterization of biomacromolecules. This unit presents a thorough discussion of the eight types of HPLC currently used, highlighting equipment and start-up procedures, recommendations for running each type of experiment, and theoretical considerations for the separation of peptides and proteins. This ...

oDays High-performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds. The evaporative laser light-scattering detector (ELSD) offers new opportunities for quantitative analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soyheans subjected to storage at high moisture conten...

background valproic acid is widely used as an anticonvulsant and mood-stabilizing drug in the treatment of epilepsy. determination of serum valproic acid is required in epilepsy therapy for efficient control of seizures. objectives in the present study, the gas chromatography method was applied to determine serum valproic acid and resulting data were compared with those obtained by high perform...

This is a review of analytical methods, such as spectrophotometry, derivative spectrophotometry and various chromatographic (gas chromatography-GC, high-performance liquid chromatography-HPLC, thin-layer chromatography-TLC, high-performance thin-layer chromatography-HPTLC, liquid chromatography-tandem mass spectrometry-LC-MS, microchip electrophoresis-MCE, capillary electrophoresis-CE) and elec...

Abbreviation Key HBSS = Hanks balanced salt solution, HPLC = high performance liquid chromatography, IL-2 = interleukin-2, NK = natural killer, SAPLIP = saposin-like protein.

the efficacy of activation methods and coupling were studied in the context of performance in batch and fixed bed binding experiments utilizing cell culture fluids or blood plasma as feedstock. conclusions were drawn regarding selection of solid phase according to pore size, rigidity, ph stability, chemistry of derivation and activation, and gross concentration of immobilized ligand required fo...