growing applications of phenylalanine dehydrogenase (phedh) enzyme in the medical and pharmaceutical industries encourages researchers to seek simple, fast and economical alternative purification methods. with goal of finding a new technique, the extraction and purification of recombinant bacillus badius phedh in polyethylene glycol 6000 (peg-6000) and ammonium sulfate aqueous two-phase systems...

C-phycocyanin from Spirulina platensis was purified in aqueous two-phase systems (ATPS) of polyethylene glycol (PEG)/potassium phosphate, varying the molar mass of the PEG. Results using a full factorial design showed that an increase in the concentration of salt and decrease in the concentration of PEG caused an increment in the purification factor for all the ATPS studied. Optimization of the...

The effects of propylene glycol (PEG) on performance, ruminal fermentation, blood glucose and insulin, carcass traits, and abundance of IGF-1 mRNA in LM and leptin mRNA in adipose tissue were examined in 20 Korean native steers, with 10 each in control and PEG-fed groups, respectively. Propylene glycol mixed with concentrate diet was provided daily at a rate of 2.5 mL/kg BW(0.75). Experimental ...

Foam thin liquid films (TLF) and monolayers at the air-water interface formed by DMPC mixed with DMPE-bonded poly (ethylene glycol)s (DMPE-PEG(550), DMPE-PEG(2000) and DMPE-PEG(5000)) were obtained. The influence of both (i) PEG chain size (evaluated in terms of Mw) and mushroom-to-brush conformational transition and (ii) of the liposome/micelle ratio in the film-forming dispersions, on the int...

Basic fibroblast growth factor (bFGF) was immobilized to hydrogel scaffolds with retention of mitogenic and chemotactic activity. The bFGF was functionalized in order to incorporate it covalently within polyethylene glycol (PEG) hydrogel scaffolds by reaction with acryloyl-PEG-NHS. Hydrogels were formed by exposing aqueous solutions of PEG diacrylate, acryloyl-PEG-RGDS, and acryloyl-PEG-bFGF to...

The distribution and excretion of I-PEG-rhIL-6 in rats were investigated by I isotope tracing method. Results showed that the concentration in the brain is lowest and highest in plasma 8 h post-injection. The results indicated that the blood was the main site of action of I-PEG-rhIL-6, which provides a basis for PEG-rhIL-6 treating of thrombocytopenia.

BACKGROUND This study was performed to develop a functional poly(D,L-lactide-co-glycolide)-poly(ethylene glycol) (PLGA-PEG)-bearing amino-active end group for peptide conjugation. METHODS AND RESULTS PLGA was preactivated following by copolymerization with PEG diamine. The resulting amphiphilic PLGA-PEG copolymer bearing 97.0% of amino end groups had a critical micelle concentration of 3.0 × ...

Polyethylene glycols (PEGs) are products of condensed ethylene oxide and water that can have various derivatives and functions. Since many PEG types are hydrophilic, they are favorably used as penetration enhancers, especially in topical dermatological preparations. PEGs, together with their typically nonionic derivatives, are broadly utilized in cosmetic products as surfactants, emulsifiers, c...

The mixture of C12E5 microemulsion with PEG have been studied by small-angle X-ray scattering and dynamic light scattering in order to determine structure and dynamic of the system. Light scattering experiment shown an exponential relaxation for pure C12E5 microemulsion that with increasing of PEG concentration in the C12E5 microemulsion, relaxation becomes non-exponential, which demonstrates t...

Water potential (Psiomega) values were determined for aqueous colloids of four molecular sizes of polyethylene glycol (PEG) using freezing-point depression and vapor-pressure deficit methods. A significant third-order interaction exists between the method used to determine Psiomega, PEG molecular size, and concentration. At low PEG concentrations, freezing-point depression measurements result i...