Anaerobic ammonia oxidizing (anammox) bacteria play an important role in transforming ammonium to nitrogen gas and contribute to fixed nitrogen losses in freshwater environments. Understanding the diversity and abundance of anammox bacteria requires reliable molecular tools, and these are not yet well established for these important Planctomycetes. To help validate PCR primers for the detection...

This study aimed at evaluating potential differences among the bacterial communities from formation water and oil samples originated from biodegraded and non-biodegraded Brazilian petroleum reservoirs by using a PCR-DGGE based approach. Environmental DNA was isolated and used in PCR reactions with bacterial primers, followed by separation of 16S rDNA fragments in the DGGE. PCR products were als...

Bacteria play an important role in the initiation and progression of periodontal diseases and are part of a biofilm, which can contain over 100 different species. The aim of the present study was to show the potential of denaturing gradient gel electrophoresis (DGGE) as a tool for the detection of clinically relevant species and to compare the results of detection by DGGE with those by PCR and ...

We investigated the structure of bacterial communities present in livestock manure-based composting processes and evaluated the bacterial succession during the composting processes. Compost samples were derived separately from swine manure, dairy manure and sewage sludge. The structure of the bacterial community was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (...

the intestinal bacterial diversity of stichopus japonicus was investigated using 16s ribosomal rna gene (rdna) clone library and polymerase chain reaction/denaturing gradient gel electrophoresis (pcr-dgge). the clone library yielded a total of 188 clones, and these were sequenced and classified into 106 operational taxonomic units (otus) with sequence similarity ranging from 88 to 100%. the cov...

The diverse bacteria encoding histidine decarboxylase gene during the fermentation of Sichuan-style sausages were investigated by culture-dependent techniques, polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE), and high-throughput sequencing. All microbial indicators exhibited advantages mixed starter culture stability microecosystem was more in inoculation group than...

Ghee is widely produced from a traditional fermented butter-like product named mashita in western Uganda. However, no detailed studies have been done to identify the microorganisms involved in mashita fermentation. The aim of this study was to identify the microorganisms present at the end of mashita ripening using culture-dependent and culture-independent techniques. The most commonly identifi...

Histomoniasis is a disease of turkeys on litter or range caused by the fragile protozoan Histomonas meleagridis, a parasite of worms, primarily spread in faeces, in Heterakis gallinarum (caecal worm) eggs or in Eisenia foetita (earthworms). Symptoms include poor feed conversion ratio (FCR), decreased body weight (BW), diarrhea, caecal and liver lesions, darkening of the facial regions and somet...

The molecular fingerprinting technique (PCR-DGGE) of microbial populations was successfully adopted for soilless culture systems. Relatively low concentrations of microorganisms in the nutrient solutions could be assessed. PCR-DGGE was applied in several experiments to compare the composition and diversity of the bacterial populations in different treatments, as well as at different locations a...

Background: The aim of the study was to analyze the performance of PCR-DGGE based assay and its applicability as a tool for the identification of bacteria in the middle ear of children with otitis media with effusion (OME). Methods: The middle ear effusions from 20 children with OME were analyzed both by bacterial culture and by 16S rDNA-gene-targeted PCR assay, DGGE fingerprinting and sequenci...