SARS-CoV-2, a single stranded positive RNA-virus, emerged in 2019 and caused global pandemic. Some infected patients develop severe complications, such as acute respiratory distress syndrome (ARDS) pulmonary fibrosis. The mechanisms leading to lung fibrosis the context of COVID-19 are still unclear. However, there correlations between this condition certain inflammatory mediators that elevated ...

Human luteal cells have been reported to express human leukocyte antigen-DR and lymphocyte functional antigen-3 on the cell surface, suggesting physiological interaction between luteal cells and T-lymphocytes through the menstrual cycle into early pregnancy. To elucidate the role of peripheral lymphocytes on corpus luteum differentiation, the effect of peripheral blood mononuclear cells (PBMC) ...

We previously reported that butyric acid, an extracellular metabolite from periodontopathic bacteria, induced apoptosis in murine thymocytes, splenic T cells, and human Jurkat T cells. In this study, we examined the ability of butyric acid to induce apoptosis in peripheral blood mononuclear cells (PBMC) and the effect of bacterial lipopolysaccharide (LPS) on this apoptosis. Butyric acid signifi...

Background: Glucose is one of the principal energy suppliers for normal cell development. Impaired glucose metabolism may lead to serious health and immune impediments including carcinogenesis. The question posed in the present work was if glucose, at several concentrations, affects cytokine production by normal human peripheral blood mononuclear cells (PBMC) and if glucose is able to modulate ...

As soon as Peripheral Blood Mononuclear Cells (PBMC) are isolated from whole blood, some cells begin dying. The rate of apoptotic cell death is increased when PBMC are shipped, cryopreserved, or stored under suboptimal conditions. Apoptotic cells secrete cytokines that suppress inflammation while promoting phagocytosis. Increased numbers of apoptotic cells in PBMC may modulate T cell functions ...

BACKGROUND During chronic inflammation, immune cells, notably Th17 cells, infiltrate the inflammatory site and interact with local mesenchymal cells. Applied to rheumatoid arthritis (RA), the aim is to study the interactions between synoviocytes and peripheral blood mononuclear cells (PBMC) with a focus on the Th17 pathway and to identify a mechanism which leads to high IL-17 secretion with an ...

INTRODUCTION Th17 cells have been implicated in the pathogenesis of rheumatoid arthritis (RA). The aim of this study was to systematically analyse the phenotype, cytokine profile and frequency of interleukin-17 (IL-17) producing CD4-positive T cells in mononuclear cells isolated from peripheral blood, synovial fluid and synovial tissue of RA patients with established disease, and to correlate c...

Abstract Human immune cell reconstitution (Peripheral Blood Mononuclear Cells; PBMC or CD34 +hematopoietic stem cell; HSC) is commonly performed in immunodeficient mouse strains, such as NCG, NSG, NOG. utilizing PBMCs a much more rapid model, allowing for T-cell within 2 weeks. Due to the occurrence of xenogeneic graft-versus-host disease (xGvHD) around 4 weeks post-engrafment, application PBMC...

Recurrent aphthous ulcerations (RAU) represent a common oral mucosal disease with altered humoral and cellular immunities. In our institution, an immunomodulating agent, levamisole, is used to treat RAU with satisfactory therapeutic effect. Tien-Hsien liquid (THL) is an extract of Chinese medicinal herbs with immunomodulating effects. To test whether THL has immunomodulating effects on antigen-...

BACKGROUND Acute myocardial infarction (AMI) is followed by post AMI cardiac remodelling, often leading to congestive heart failure. Homing of c-kit+ endothelial progenitor cells (EPC) has been thought to be the optimal source for regenerating infarcted myocardium. METHODS Immune function of viable peripheral blood mononuclear cells (PBMC) was evaluated after co-culture with irradiated apopto...