INTRODUCTION Among the bacterial causes, diarrheagenic Escherichia coli (DEC) is the most important etiologic agent of childhood diarrhoea and represents a major public health problem in developing countries. New evidence suggests that major differences in virulence among groups of DEC pathotypes may be related to the presence of specific pathogenicity islands (PAIs). METHODOLOGY Multiplex an...

Escherichia coli O103, harbored in the hindgut and shed in the feces of cattle, can be enterohemorrhagic (EHEC), enteropathogenic (EPEC), or putative non-pathotype. The genetic diversity particularly that of virulence gene profiles within O103 serogroup is likely to be broad, considering the wide range in severity of illness. However, virulence descriptions of the E. coli O103 strains isolated ...

pathogenic diversity in didymella rabiei in ilam and kermanshah provinces was assessed with the objective of finding out about the existence of pathotypes. one hundred isolates from different farms of chickpea growing regions in these provinces were collected in 2007. isolates were arranged into ten different groups based upon their sampling regions. one isolate was chosen from anyone of the te...

The molecular revolution in clinical and diagnostic microbiology has been fueled by such innovative technologies as polymerase chain reaction (PCR), matrix-assisted laser desorption ionization-time of flight mass spectroscopy (MALDI-TOF) and 16S rRNA and full genome sequencing [1,2]. This revolution has spurred numerous advancements in the field of microbiology in the area of rapid diagnostics,...

One approach to the accurate determination of the pathogenic potential (pathotype) of isolated Escherichia coli strains would be through a complete assessment of each strain for the presence of all known E. coli virulence factors. To accomplish this, an E. coli virulence factor DNA microarray composed of 105 DNA PCR amplicons printed on glass slides and arranged in eight subarrays corresponding...

In North America there are two generally recognized pathotypes (pathotypes 1 and 2) of the fungus Entomophaga grylli which show host-preferential infection of grasshopper subfamilies. Pathotype 3, discovered in Australia, has a broader grasshopper host range and was considered to be a good biocontrol agent. Between 1989 and 1991 pathotype 3 was introduced at two field sites in North Dakota. Sin...

Part of the Thesis of the first author. Universidade Federal de Viçosa. Viçosa MG. 2004. *Present Address: Laboratório de Microbiologia Agrícola, ICA, Universidade Federal Rural da Amazônia, 66077-530, Belém, PA, Brazil ABSTRACT Genetic and phenotypic structure of Magnaporthe oryzae populations of two upland rice cultivars was determined. Monoconidial isolates were obtained from rice blast aff...

Foodborne diseases occur worldwide, including those acquired through the consumption of contaminated meat. This study aimed to investigate the prevalence of enterotoxigenic Staphylococcus aureus, Salmonella and Escherichia coli pathotypes in different meat types. Forty meat samples fresh (n=35) and frozen (n=5) were purchased from local markets in Jenin district, Palestine. Multiplex PCR was us...