نتایج جستجو برای: passaged

تعداد نتایج: 1753  

Journal: :Antimicrobial agents and chemotherapy 2015
Liana C Chan Li Basuino Binh Diep Stephanie Hamilton Som S Chatterjee Henry F Chambers

The role of mecA mutations in conferring resistance to ceftobiprole and ceftaroline, cephalosporins with anti-methicillin-resistant Staphylococcus aureus (MRSA) activity, was determined with MRSA strains COL and SF8300. The SF8300 ceftaroline-passaged mutant carried a single mecA mutation, E447K (E-to-K change at position 447), and expressed low-level resistance. This mutation in COL conferred ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2003
Eain Murphy Dong Yu Jane Grimwood Jeremy Schmutz Mark Dickson Michael A Jarvis Gabriele Hahn Jay A Nelson Richard M Myers Thomas E Shenk

Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs w...

Journal: :Comparative biochemistry and physiology. B, Comparative biochemistry 1984
D J Howse J M Potter D I Grove

An anticoagulant has been purified from the body fluid of Ascaris suum by sequential passage through Sephadex G-50, CM-cellulose and Sephadex G-25 columns then treated with 2 M NaCl, passaged through a Sephadex G-25 column, separated from the phosphate buffer by precipitation of the latter with the CaCl2, then passaged through a Sephadex G-10 column in water. In the body fluid of the worm, the ...

2001
R. R. Woolaston R. L. Elwin

A genetically diverse population of Haemonchus contortus was serially passaged through Merino hosts bred for either increased or decreased resistance to the parasite. After 30 parasite generations, the resulting nematode sub-populations were compared with each other and with the foundation population, by infecting lines of the selected sheep and measuring the resulting faecal egg counts (FECs) ...

2015
Nathan D. Grubaugh Darci R. Smith Doug E. Brackney Angela M. Bosco-Lauth Joseph R. Fauver Corey L. Campbell Todd A. Felix Hannah Romo Nisha K. Duggal Elizabeth A. Dietrich Tyler Eike Jennifer E. Beane Richard A. Bowen William C. Black Aaron C. Brault Gregory D. Ebel Marco Vignuzzi

Within hosts, RNA viruses form populations that are genetically and phenotypically complex. Heterogeneity in RNA virus genomes arises due to error-prone replication and is reduced by stochastic and selective mechanisms that are incompletely understood. Defining how natural selection shapes RNA virus populations is critical because it can inform treatment paradigms and enhance control efforts. W...

Journal: :The Journal of endocrinology 2004
Yan Qiu Michael J S Langman Margaret C Eggo

Epidemiological studies show a strong link between postmenopausal hormone replacement therapy and decreased incidence of colorectal cancer. The colon cancer cell line, COLO 205, develops sensitivity to 17beta-oestradiol (E(2)) in apoptosis assays with increasing passage number (>40), and we hypothesised that genes selectively regulated in multiply passaged cells were likely to be important in E...

Journal: :Journal of virology 1998
R M Welsh C L O'Donnell D J Reed R P Rother

Human sera contain high levels of natural antibody (Ab) to Galalpha1-3Gal, a terminal glycosidic structure expressed on the surface of cells of mammals other than Old World primates. Incorporation of this determinant onto retroviral membranes by passage of viruses in cells encoding alpha-1-3-galactosyltransferase (GT) renders retroviruses sensitive to lysis by natural Ab and complement in norma...

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