Aqueous two-phase systems (ATPS) have  emerged as a powerful extraction method for the downstream processing of bio-molecules. The aim of this work was to investigate the possibility of utilizing ATPS for the separation of recombinant Bacillus sphaericus phenylalanine dehydrogenase (PheDH). Polyethylene glycol (PEG) and ammonium sulfate systems were selected for our experi...

To accelerate the calculation speed for simulating a biological system, we proposed a novel simulation method, the two-phase partition method, which calculated molecular processes at a higher speed than any other proposed method. This method divides a biological system, which can be described by chemical reaction equations, into two-phases: the binding and reaction phases. We demonstrated the c...

The Kuramoto model describes the collective dynamics of a system of coupled oscillators. An α-Kuramoto partition is a graph partition induced by the Kuramoto model, when the oscillators include a phase frustration parameter. We prove that every equitable partition is an α-Kuramoto partition, but that the converse does necessarily not hold. We give an exact characterisation of α-Kuramoto biparti...

We propose a method to find the QCD critical point at finite density calculating the canonical partition function ZC(T, N) by Monte-Carlo simulations of lattice QCD, and analyze data obtained by a simulation with two-flavor p4-improved staggered quarks with pion mass mπ ≈ 770MeV. It is found that the shape of an effective potential changes gradually as the temperature decreases and a first orde...

This report focus on phase behavior of polyethylene glycol (PEG)4000/ phosphate/ guanidine hydrochloride/ water system at different guanidine hydrochloride concentrations and pH. The binodal of the systems was displaced toward higher concentrations of the components with increasing guanidine hydrochloride concentrations. The partition coefficient of guanidine hydrochloride was near unity and in...

This report focus on phase behavior of polyethylene glycol (PEG)4000/ phosphate/ guanidine hydrochloride/ water system at different guanidine hydrochloride concentrations and pH. The binodal of the systems was displaced toward higher concentrations of the components with increasing guanidine hydrochloride concentrations. The partition coefficient of guanidine hydrochloride was near unity and in...

Nested Sampling (NS) is a powerful athermal statistical mechanical sampling technique that directly calculates the partition function, and hence gives access to all thermodynamic quantities in absolute terms, including absolute free energies and absolute entropies. NS has been used predominately to compute the canonical (NVT) partition function. Although NS has recently been used to obtain the ...