Fixation of tissues with formalin results in well-preserved morphology but to a high degree leads to degradation of nucleic acids, which substantially constricts the spectrum of applicable molecular techniques. The novel HOPE-fixative with subsequent paraffin embedding, as an alternative to formalin, has been shown to result in a morphological preservation comparable to formalin-fixed, paraffin...

bedding of vitrectomized specimens requires a large number of cells, as in cases of vitreous hemorrhage, endophthalmitis, or diabetic retinopathy. In these instances, a considerable amount of cells will be lost. Paraffin embedding of vitrectomized intraocular fluids using the fibrin and paraffin method has the following important advantages. Specimens with small numbers of cells from patients w...

Immunohistochemistry (IHC) is a routinely used technique in clinical diagnosis of pathological conditions and in basic research. It combines anatomical, immunological, and biochemical methods and relies on the specific binding of an antibody to an antigen. Using the technique with mineralised tissues is more complicated than with soft tissues. This can in most cases be overcome by demineralisin...

Submit Manuscript | http://medcraveonline.com of tissue samples [1,2]. Rationalisation of biomarkers using the conventional histopathological procedure is time consuming, laborious and pricey especially, when several markers are to be tested on various specimens. So, TMA is expected to surmount these substantial problems [3]. Tissue microarrays are produced by harvesting cylindrical tissue core...

The histological processing of teeth is highly complicated because of containing both mineralized hard tissues and soft tissues. Depending on the type of decalcification agents used in processing, mild-to-severe deterioration in the tissue structure and inadequacies on clear staining of details by the histological stain may be observed. This study aims to compare the histological staining diffe...

Improved cytomorphology of semithin resin sections over paraffin wax embedded sections may be important in diagnostic haematopathology. However, resin embedding can make immunohistochemical antigen detection or DNA isolation for clonal gene rearrangement assays difficult. This review describes the processing of bone marrow biopsies using buffered formaldehyde based fixation and epoxy resin embe...

In both groups, testicular biopsies were performed during orchiopexy. Each specimen was fixed in Bouin’s solution and processed for paraffin embedding. The sections were stained with hematoxylin–eosin and examined under 300 magnifications. Observed fertility indexes were calculated by counting the number of spermatogonia per tubule in 50 tubules examined at 300 magnifications and averaging the ...

The production of 0.4mu thick, full-length sections from renal tissue taken by needle biopsy is achieved by formalin and osmic acid fixation and embedding in epoxy resin as for ultramicrotomy. The marked improvement in histological clarity by using this technique is illustrated by comparison with material embedded in paraffin.

transaminase, glutamate pyruvate transaminase, alkaline phosphatase (ALP) and serum bilirubin (total and direct) were determined (Table 1). Liver was excised quickly fixed in 10% formalin and then fixed in bovine solution, they were processed for paraffin embedding following the standard micro technique. Sections of liver were stained with haematoxylin-eosin and were observed microscopically fo...