Freshwater sponges (Porifera: Spongillida) are sessile invertebrates with skeletons composed of siliceous elements termed spicules. Sponge spicules (megascleres, microscleres, and gemmuloscleres) characterized by widely varying sizes shapes. These well-preserved in lacustrine, wetland, riverine sediments hold significant ecological limnological information that can be applied as diagnostic tool...