The genus Hyoscyamus belongs to the tribe Hyoscyameae Miers of Solanaceae family. Variation in protein bands elaborates the relationship among the collections from various geographical regions. In this study the seed storage protein diversity of 19 accessions of Hyoscyamus (H. niger, H. reticulatus and  H. pusillus) from West Azerbaijan (Iran) was investigate...

The aim of the study was to describe the urinary electrophoretic pattern of dogs with heartworm disease.Urine samples from 15 heartworm-infected and 15 healthy dogs were taken. Urinary specific gravity, urinaryprotein concentration and the urine protein/creatinine (U P/C) ratio were determined. Urine proteins werefractionated using SDS-PAGE. Results showed statistically significant differences ...

We have modified one of the most useful methods of protein separation; namely, two dimensional gel electrophoresis (2-DE). This modified version of 2-DE is not only simpler and easier but also faster than all the currently available methods. In this method, isoelectric focusing is carried out in the first dimension using a vertical sodium dodecyl sulfate polyacrylamide gel electrop...

background: it seems that the success of vaccination for cancer immunotherapy such as dendritic cell (dc) based cancer vaccine is hindered through a powerful network of immune system suppressive elements in which regulatory t cell is the common factor. foxp3 transcription factor is the most specific marker of regulatory t cells. in different studies, targeting an immune response against regulat...

bovine ephemeral fever (bef) is an acute epidemic disease in cattle and water buffalo, spanning tropical and subtropical zones of asia, australia, and africa. in recent years, bef has been distributed in many provinces of iran and caused economic losses. in this study the sequence encoding the g1 epitope of bovine ephemeral fever virus (befv) g glycoprotein was amplified by pcr, ligated into th...

the aim of the study was to describe the urinary electrophoretic pattern of dogs with heartworm disease.urine samples from 15 heartworm-infected and 15 healthy dogs were taken. urinary specific gravity, urinaryprotein concentration and the urine protein/creatinine (u p/c) ratio were determined. urine proteins werefractionated using sds-page. results showed statistically significant differences ...

Background: Xanthomonas citri subsp. citri (Xcc), the causative agent of bacterial citrus canker, has affected citriculture worldwide. Varieties of means have been used to minimize its devastating effects, but no attention has been given to bacteriocins. Objectives: Here and for the first time, we report the isolation and characterization of two novel bacteriocins. Materials and Methods: Secret...

background : there are several methods, such as vaccination, to control visceral leishmaniasis. although there is no efficient vaccine, it seem dna vaccination with stimulates both cellular and humoral immunity apparently is the best way. the aim of this study was cloning and expression of lack gene, a 36kd protein, as a can-didate protein for vaccination against iranian l.infantum. methods: ir...

background: xanthomonas citri subsp. citri (xcc), the causative agent of bacterial citrus canker, has affected citriculture worldwide. varieties of means have been used to minimize its devastating effects, but no attention has been given to bacteriocins.objectives: here and for the first time, we report the isolation and characterization of two novel bacteriocins.materials and methods: secretom...

The aim of this study was to construct, expression of a novel recombinant chimeric protein consisting of Pyruvate dehydrogenase beta subunit (PDHB) and high antigenic region of integral membrane lipoprotein P80 of Mycoplasma agalactiae as a potential diagnostic tool. The full-length sequence of pdhb and a portion of antigenic regions of P80 were selected and analyzed by CLC ma...