The department of internal medicine at the University of Strassburg in the 1880s was noted for its investigations into intestinal worms. Adolf Kussmaul, the director, asked two young assistants, Arnold Cahn and Paul Hepp, to treat patients with naphthalene as it had been used elsewhere as an internal antiseptic. The young doctors were disappointed with the initial results, but Hepp persevered w...

The problem of the biochemical activation steps required to elicit carcinogenicity to rat liver by yV-2-fluorenylacetamide was investigated by bioassay, competitive inhibition, and biochemical techniques. Acetanilide inhibits not only the hepatocarcinogenicity of JV-2-fiuorenylacetamide but also that of the carcinogenic W-hydroxylated metabolite. With the parent compound, the inhibition was tra...

This paper reviews our experimental and theoretical efforts toward understanding vibrational self-trapping of the amide I and N-H mode of crystalline acetanilide (ACN), other similar hydrogen-bonded crystals, as well as of model peptides. In contrast to previous works, we used nonlinear IR spectroscopy as the experimental tool, which is specifically sensitive to the anharmonic contributions of ...

A thorough, systematic study into the effect that structurally related impurities have on both process and product quality during crystallization of an active pharmaceutical ingredient is presented. The presence acetanilide metacetamol influences paracetamol. Where high concentrations either impurity were present in feed, recovery decreased by up to 15%. Acetanilide included final through adsor...

The problem of the biochemical activation steps required to elicit carcinogenicity to rat liver by yV-2-fluorenylacetamide was investigated by bioassay, competitive inhibition, and biochemical techniques. Acetanilide inhibits not only the hepatocarcinogenicity of JV-2-fiuorenylacetamide but also that of the carcinogenic W-hydroxylated metabolite. With the parent compound, the inhibition was tra...

The unidirectional circulation of an enzyme round a working cycle can be understood in terms of the formation and decay of a soliton that acts as a ligand for the protein and for which the surrounding heat bath serves as a sink. An experimental confirmation of this interpretation is suggested in which the soliton is of the Davidov type and is created by infrared photon absorption as in recent e...

A microsomal deacetylase that catalyses the deacetylation of the O-glucoside of N-hydroxyacetanilide (GHA) was purified from guinea-pig liver. The activity was located exclusively in the microsomes and not detected in the cytosol. The purified GHA deacetylase was a trimeric protein with a molecular mass of 160+/-10 (S.D.) kDa composed of subunits of 53+/-2 kDa; its pI was 4.7. The N-terminal am...