Two experiments were conducted to study the efficiency of ovine follicular fluid (OFF) and age ewe on in vitro nuclear maturation Awassi sheep oocytes (Experiment 1) subsequent stages early embryos their quality 2). The OFF has been added basic medium (TCM-199) with three different levels (A: 0% (Control group), B:15%, C:25% D:50%) while ages ewes determined into two main groups (> 2 1-2 yea...

The present study was designed to investigate the effect of supplementing of protein additives (PA) in forms of 10% ovine amniotic fluid (OAF), 10% fetal bovine serum (FBS) and 10% sheep serum (SS), individually to culture media (TCM-199 or RPMI-1640) on in vitro nuclear maturation (IVM) of sheep oocytes. In addition, the effect of seasonal variations on the yield and quality of recovered sheep...

BACKGROUND Leptin, as a 16 kDa adipokine, is a pleiotropic cytokine-like hormone that primarily secreted from adipose tissue. It also involves in the regulation of energy homeostasis, neuroendocrine function, immunity, lipid and glucose homeostasis, fatty acid oxidation, angiogenesis, puberty and reproduction. The aim of this study was to investigate the effects of in vitro addition of leptin t...

The present study was aimed to study the effect of an ovine follicular fluid peptide on ovarian follicle and good oocyte numbers and weights of ovary, uterus, liver, pancreas and kidney in rats, R. norvegicus. A 30.1 kDa peptide was isolated from ovine follicular fluid by ammonium sulphate precipitation and then gel filtration. The peptide was tested at various levels in normal (22 and 36 day-o...

Pronuclear development was used to measure the effects on ovine oocytes of altering follicular steroidogenesis during maturation in vitro. Follicular steroid secretion was altered using enzyme inhibitors and exogenous steroid supplementation. Abnormalities induced during maturation were measured 24 h after tranfer of oocytes to the oviducts of inseminated hosts. The presence throughout maturati...

BACKGROUND This study was aimed to assess the effects of angiotensin II (Ang II) supplementation to the In Vitro Maturation (IVM) and In Vitro Culture (IVC) media of vitrified-warmed ovine oocytes on their developmental competence and expression of Na(+)/K(+)/ATPase in resulting embryos. METHODS The slaughterhouse-derived immature oocytes (n=1069) were randomly distributed into four experimen...

Background: In this study we evaluate the effects of different doses of GDF9 with fixed dose of BMP15 on quality of in vitro maturated sheep oocytes. Materials and Methods: Good ovine Cumulus-oocyte complexes (COCs) were first transferred to Pre-IVM medium, and then to three IVM mediums supplemented with 10%v/v BMP15 and: 1) 175 ng/ml 2) 131 ng/ml or 3) 219 ng/ml GDF9. After maturation period, ...

In order to evaluate the effect of O2 tension on the in vitro maturation (IVM) and fertilization (IVF) process, 11 replicates of OPU/IVP were performed using 1092 viable oocytes obtained from 48 Bos indicus embryo donors of commercial herds. Cumulus-oocyte complexes (COCs) from each donor were homogeneously and randomly distributed across two treatments. The IVM was performed in TCM-199 with ov...

The recovery of immature oocytes followed by in vitro maturation, fertilization and culture (IVMFC) allows the rescue of biological material of great genetic value for the establishment of genetic resource banks of endangered species. Studies exist on sperm cryopreservation of endangered Mohor gazelle (Gazella dama mhorr), but no work has been carried out yet on oocyte collection, fertilization...