A single-tube duplex nested polymerase chain reaction (sdn-PCR) was developed for the detection of and discrimination between ovine herpesvirus-2 (OvHV-2) and alcelaphine herpesvirus-1 (AIHV-1). These viruses respectively cause sheep- and wildebeest-associated malignant catarrhal fever (SA-MCF and WA-MCF). In the first step of the sdn-PCR, two primers with high annealing temperatures based on c...

Mucoid nasal discharge, loss of weight, decreased milk production, diarrhoea, salivation, dyspnoea, fever, lacrimation, bilateral corneal opacity and bloody urine were observed in cattle located in the Arbe Gona district of southern Ethiopia. The disease was associated with a high case fatality rate: diseased cattle died within four to five days after showing clinical signs. The clinical presen...

Malignant catarrhal fever (MCF) is a disease causing fatal outcome in cattle and generates economic losses worldwide. This study aims to detect the cause of Balinese showing clinical signs such as high fever, serous ocular mucopurulent nasal discharges, enlargement pre-scapularis pre-femoralis lymphnodes. These were previously housed 50 meters away from flock sheep which brought Sabu Island 3 m...

Malignant catarrhal fever (MCF) is a fatal lymphoproliferative disease of cattle and other ungulates caused by the ruminant gamma-herpesviruses alcelaphine herpesvirus 1 (AlHV-1) and ovine herpesvirus 2 (OvHV-2). These viruses cause inapparent infection in their reservoir hosts (wildebeest for AlHV-1 and sheep for OvHV-2), but fatal lymphoproliferative disease when they infect MCF-susceptible h...

A fatal enteric syndrome was identified in American bison (Bison bison) at a large feedlot in the American Midwest in early 1998. An estimated 150 bison died of the syndrome between January 1998 and December 1999. The syndrome was identified as malignant catarrhal fever (MCF), primarily the alimentary form. Clinical onset was acute, and most affected bison died within 1-3 days; none recovered. ...

Malignant catarrhal fever (MCF) caused by OvHV-2 occurred in ranch bison herds separated by significant distances from feedlot lambs. Mortality rates correlated with distances: 17.5%, 6.1%, and 0.43% at approximately 1.6, 4.2, and 5.1 km, respectively. The study further defines the importance of distance of species separation for MCF control.

Infection with Ovine herpesvirus 2 (OvHV-2) in healthy cattle, swine, sheep, and goats was investigated on 43 selected Norwegian farms; of which, 41 (95%) had experienced outbreaks of malignant catarrhal fever (MCF) in cattle and/or swine during the preceding 5 years. Two of the farms had no history of MCF and were included for control purposes. Blood samples from 384 cattle, 40 sows, 75 sheep,...