Formalin-fixed paraffin-embedded (FFPE) breast tumor tissues are readily available and represent a largely untapped, vast resource for molecular profiling of clinical samples with long-term follow-up data. We have optimized the conditions and parameters that result in the preparation of total RNA that is of the necessary quality for use in the DASL (cDNA-mediated annealing, selection, extension...

To develop a portable point-of-care system based on biosensors for common infectious diseases such as urinary tract infection, the sensing process needs to be implemented within an enclosed fluidic system. On chip sample preparation of clinical samples remains a significant obstacle to achieving robust sensor performance. Herein AC electrokinetics is applied in an electrochemical biosensor cass...

Introduction: The endemic occurrence of new strains of tuberculosis around the globe has initiated new research on developing diagnostic radiopharmaceutical based on antibiotics. Methods: Tc-99m labeled streptomycin was prepared using freshly milked TcO4 and various concentrations of streptomycin, stannous chloride at various temperatures in high yield radiochemical purity shown by HPLC/RTLC. R...

one of the important technical obstacles in the study of many filamentous fungi is the development of efficient transformation system. transformation of filamentous fungi is difficult because they have a cell wall and for most frequently used approaches preparing of protoplast is necessary. protoplast preparation is batch-dependent and often frustrating work. in this study, a modified method wa...

PURPOSE We aimed to investigate the effect of key imaging parameters on the accuracy of apparent diffusion coefficient (ADC) maps using a phantom model combined with ADC calculation simulation and propose strategies to improve the accuracy of ADC quantification. METHODS Diffusion-weighted imaging (DWI) sequences were acquired on a phantom model using single-shot echo-planar imaging DWI at 1.5...

The cycling parameters for BOX A1R PCR were modified and the primer-specific annealing temperature was optimized. Using Trout Lake Escherichia coli isolates, four different template preparation methods were compared. The cell washing, heat lysis, and DNA isolation methods all gave clear and bright band patterns with good reproducibility. The cell washing template preparation method for direct u...

cobalt is widely used in different industrial processes for production of various synthetic materials. for assessment of human exposure to toxic metal of co (ii), environmental and biological monitoring are essential processes, in which, preparation of samples is one of the most time-consuming and error-prone aspects prior to instrumental analysis. the aim of this study was to achieve optimum f...

this work aimed to prepare a nanoemulsion preparation containing budesonide and assess its aerodynamic behavior in comparison with suspension of budesonide. in-vitro aerodynamic performance of the corresponding micellar solution (ie. nanoemulsion preparation without oil) was investigated too. nanoemulsions of almond oil containing budesonide, as a hydrophobic model drug molecule, were prepared ...