Selective-plane illumination microscopy (SPIM) provides unparalleled advantages for the volumetric imaging of living organisms over extended times. However, the spatial configuration of a SPIM system often limits its compatibility with many widely used biological sample holders such as multi-well chambers and plates. To solve this problem, we developed a high numerical aperture (NA) open-top co...

Experiments were conducted with two commonly grown mungbean varieties viz., M-28 and 6601, in open top chambers and ambient field conditions with the aim of ascertaining the effects of air pollution on yield. The 8-h daily mean ozone concentrations were 41-73 nl litre. A considerable reduction of 47.06% and 51.12% in seed yield for M-28 and 6601, respectively were found which are substantially ...

We measured growing season soil CO, evolution under elevated atmospheric CO, and soil nitrogen (N) additions. Our objectives were to determine treatment effects, quantify seasonal variation, and determine regulating mechanisms. Elevated CO, treatments were applied in opentop chambers containing 3-year-old ponderosa pine ponderosa Dougl. ex Laws.) seedlings. Nitrogen applications were made annua...

The second UN/ECE ICP-Forests Intercalibration Course on the Assessment of Ozone Injury on European Tree Species was carried out in August 2001 at Lattecaldo (Canton Ticino, CH) and Moggio (Lombardy, I). Forty-eight experts from several European countries participated in the exercises and assessed visible symptoms of ozone injury both in open-top chambers (OTC) (Lattecaldo) and under open field...

As atmospheric CO2 concentration increases, many experiments have been carried out to study effects of CO2 enrichment on litter decomposition and nutrient release. However, the result is still uncertain. Meanwhile, the impact of CO2 enrichment on nutrients other than N and P are far less studied. Using open-top chambers, we examined effects of elevated CO2 and N addition on leaf litter decompos...

Responses to elevated CO"2 have not been measured for natural grassland ecosystems. Global carbon budgets will likely be affected by changes in biomass production and allocation in the major terrestrial ecosystems. Whether ecosystems sequester or release excess carbon to the atmosphere will partly determine the extent and rate that atmospheric CO"2 concentration rises. Elevated CO"2 also may ch...