seth4 coding sequence with 2013 bp is a member of gene family expressed in gametophytic tissues of arabidopsis thaliana. this fragment was pcr amplified using kod hi fi dna polymerase enzyme. this fragment was cloned into pgbkt7 bate vector and transformed e. coli dh5? cells containing vector were selected on lb medium containing kanamycin. finally, pgbkt7-seth4 bate was transformed into yeast ...

Fault diagnosis has always been an essential aspect of control system design. This is necessary due to the growing demand for increased performance and safety of industrial systems is discussed. Support vector machine classifier is a new technique based on statistical learning theory and is designed to reduce structural bias. Support vector machine classification in many applications in v...

The pLE2SCX vector was developed for the stable expression of exogenous genes in the protozoan parasite Leishmania. The pLE2SCX construct contains three independent selection markers: herpes simplex virus thymidine kinase (HSV-TK), cytosine deaminase (CD) and streptothericin acetyltransferase gene (sat) in multiple cloning site, flanking by 5’ and 3’ untranslated regions of the previously clone...

knowing that adenoviral vectors could initiate innate immunity, the ability of e1-deleted recombinant adenovirus (ad-e1δ) in induction of b7.1 and il-2 molecules was studied. methods: the expression of green fluorescent protein in c1498 cells following transfection of these cells with adenovirus green fluorescent protein vector confirmed the ability of adenovirus vectors in infecting the cells ...

Рассматриваются безвихревые векторные поля на поверхности, заданной уравнением $a=z+\alpha(x,y,t)=0$. Изучаются условия, при выполнении которых линии таких полей располагаются этой поверхности. Получены достаточные условия существования гармонического векторного с такими векторными линиями. Изучена переопределенная система уравнений в частных производных, решение которой обеспечивает получение ...