Recovery of metabolites from fermentation broth by solvent extraction can be used to optimize fermentation processes. End-product reutilization, low product concentration, large volumes of fermentation broth and the requirements for large bioreactors, in addition to the high cost largely contributed to the decline in fermentative 2, 3-butanediol production. Extraction can ...

AimThe aim of this study was to evaluate biodegradable cationic surfactants based on lysine.MethodsLysine esterified with cholesterol, oleyl alcohol and 1-decanol resulting in cholesteryl lysinate (CL), (OL) decyl (DL). Esters were investigated regarding their log Dn-octanol/water, critical micelle concentration (CMC) biodegradability. Hemolytic potential CL, OL, DL the already established hexa...

The behaviour of ultrasonic wave propagation through liquid solution has become an important tool for studying physical and chemical properties of liquid solutions. The density, viscosity and ultrasonic velocity of of cholesteryl oleyl carbonate have been studied. From the experimental data various thermo acoustical parameters such as free volume, internal pressure and Rao’s constant and Wada’s...

The surface properties of fatty acid and fatty acid-alcohol mixtures were examined at 22-24 degrees C. At pH 12, sodium stearate forms a rigid surface film that generates an equilibrium spreading pressure of 16.5 dynes/cm. At pH 12, stearate-alkaline earth cation films collapse at the air-water interface and do not generate significant equilibrium spreading pressures. The rate of film collapse ...

The objective of this study was to optimize continuous acetone-butanol-ethanol (ABE) fermentation using a two stage chemostat system integrated with liquid-liquid extraction of solvents produced in the first stage. This minimized end product inhibition by butanol and subsequently enhanced glucose utilization and solvent production in continuous cultures of Clostridium acetobutylicum B 5313. Dur...

Enzymatic synthesis of palm oil esters (POE) was carried out via alcoholysis of palm oil (PO) and oleyl alcohol (OA) catalyzed by Lipozyme TL IM. The optimum reaction conditions were: temperature: 60 °C; enzyme load: 24.7 wt%; substrate ratio: 1:3 (PO/OA), impeller speed: 275 rpm and reaction time: 3 h. At the optimum condition, the conversion of POE was 79.54%. Reusability study showed that Li...

The present study reports the results of a combined computational and site mutagenesis study designed to provide new insights into the orthosteric binding site of the human M3 muscarinic acetylcholine receptor. For this purpose a three-dimensional structure of the receptor at atomic resolution was built by homology modeling, using the crystallographic structure of bovine rhodopsin as a template...

Long-chain acyl-coenzyme A (CoA) compounds (palmityl, stearyl, and oleyl) were found to be potent inhibitors of acetyl-CoA synthetase (ACS) of Saccharomyces cerevisiae strain LK2G12 from aerobic, but not from nonaerobic, cells. The effectiveness of the inhibitors of the aerobic enzyme was in the following order: palmityl-CoA < stearyl-CoA < oleyl-CoA. Short-chain acyl-CoA compounds (propionyl, ...

Engkabang Fat Esters are produced from the alcoholysis reaction of oleyl alcohol and its fat using enzyme as biocatalyst. The work has been previously carried out mainly on the optimization of Engkabang Fat esters production in organic solvents. However, the effect of impeller speed-substrate molar ratio has yet to be discussed in detail. In the present paper, the influences of impeller speed a...

This work reports on a solvent selection for the liquid–liquid extraction of p-coumaric acid produced by an engineered strain Saccharomyces cerevisiae. The is key point processes and this describes simple strategy to choose suitable in situ or stream product recovery (ISPR) process during bioconversion. ISPR allow limit inhibition caused end-products accumulation fermentation medium. consists s...