RNA expression profiling of obligately parasitic plant microbes is hampered by the requisite interaction of host and parasite. This can be especially problematic in the case of powdery mildews, such as Erysiphe necator (syn. Uncinula necator), which grow superficially but tightly adhere to the plant epidermis. We developed and refined a simple and efficient technique in which nail polish was us...

Powdery mildew, caused by the fungus Erysiphe (Uncinula) necator is a grapevine disease of nearly universal importance, often requiring 10 to 15 annual fungicide applications for its control. In the field, disease severity on fruit and leaves provided good sunlight exposure was typically twoand sevenfold lower, respectively, than on those in the shade. Controlled-environment studies showed that...

Abstract Grape powdery mildew caused by Erysiphe ne ca tor is a limiting factor in grape production. In order to develop effective management strategies, the factors influencing survival of fungus need be identified and characterized accordingly. For this purpose, we focused on effect weather conditions during overwintering viability ascospores. spring 2017 2018, leaf litter bark samples were c...

Powdery mildew ( Erysiphe necator ) is one of the most common and economically significant diseases grapes. Currently, main method controlling disease pesticide treatment. Breeding resistant varieties necessary to reduce chemical treatments. a number grape resistance genes powdery DNA markers for identification allelic status these are known. In study determine presence loci Ren3 Ren9 , 25 geno...