The objective of this work was to molecularly and genotypically characterize test the inhibitory activity six colicinogenic Escherichia coli strains (ColEc) their partially purified colicins against STEC O157:H7 isolated from clinical human cases. Inhibition tests demonstrated these O157:H7. By PCR it possible detect Ia, E7, B microcins M, H47, C7, J25. genome sequencing two selected ColEc stra...

g 272486 t 269978 t 269979 nc O55/O157 rec01 t CB9615 ++ t g g t g g g t g g a 272489 c 269981 c 269982 nc O55/O157 rec01 c CB9615 ++ c a a c a a a c a a g 272507 c 269999 c 270000 nc O55/O157 rec01 c CB9615 ++ c g g g g g g c g g c 272519 t 270011 t 270012 nc O55/O157 rec01 c O157 ++++ c c c c c c c t c c t 272540 c 270032 c 270033 nc O55/O157 rec01 c CB9615 + g c t t c t t c c g t 272550 c 27...

Although cattle develop humoral immune responses to Shiga-toxigenic (Stx+) Escherichia coli O157:H7, infections often result in long-term shedding of these human pathogenic bacteria. The objective of this study was to compare humoral and cellular immune responses to Stx+ and Stx- E. coli O157:H7. Three groups of calves were inoculated intrarumenally, twice in a 3-week interval, with different s...

BACKGROUND Verotoxigenic E. coli (VTEC) is the cause of severe gastrointestinal infection especially among infants. Between 10 and 20 cases are reported annually to the National Infectious Disease Register (NIDR) in Finland. The aim of this study was to identify explanatory variables for VTEC infections reported to the NIDR in Finland between 1997 and 2006. We applied a hurdle model, applicable...

Characterization of an Escherichia coli O157 strain collection (n = 42) derived from healthy Hungarian cattle revealed the existence of diverse pathotypes. Enteropathogenic E. coli (EPEC; eae positive) appeared to be the most frequent pathotype (n = 22 strains), 11 O157 strains were typical enterohemorrhagic E. coli (EHEC; stx and eae positive), and 9 O157 strains were atypical, with none of th...

Shiga toxin-producing Escherichia coli (STEC) are significant public health threats. Although STEC O157 are recognized foodborne pathogens, non-O157 STEC are also important causes of human disease. We characterized 10 O157:H7 and 15 non-O157 clinical STEC derived from British Columbia (BC). Eae, hlyA, and stx were more frequently observed in STEC O157, and 80 and 100% of isolates possessed stx₁...

Shiga toxin producing Escherichia coli O157 and non-O157 serogroups are known to cause serious diseases in human. However, research on the persistence of E. coli non-O157 serogroups in preharvest environment is limited. In the current study, we compared the survival behavior of E. coli O157 to that of non-O157 E. coli strains in agricultural soils collected from three major fresh produce growin...