Nuclear factor-kappa B (NF-kappa B) is a ubiquitously expressed cellular factor regulated by the cytoplasmic factor inhibitor protein kappa B alpha (I kappa B alpha). Activation of NF-kappa B by cytokines, including tumor necrosis factor-alpha (TNF-alpha), requires the phosphorylation and degradation of I kappa B alpha. An anti-apoptotic role for NF-kappa B has recently been suggested. In the p...

Abbreviations: AM, acetoxymethylester; BSA, bovine serum albumin; DMEM, Dulbecco’s modified Eagle medium; FITC, fluorescein isothiocyanate; GM-CSF, granulocyte-macrophage colony-stimulating factor; HBSS, Hanks’ balanced salt solution; ICAM-1, intercellular adhesion molecule-1; LSC, laser scanning cytomety; NF-κB, nuclear factor-kappa B; PBS, phosphate buffered saline; PI, propidium iodide; PLP,...

Tumor necrosis factor (TNF) is known to induce the activation of a nuclear transcription factor, nuclear factor kappa B (NF-kappa B), in a wide variety of cell types. The post-receptor binding events that culminate in TNF-dependent NF-kappa B activation are not understood. To dissect this pathway, we developed a reconstitution system consisting of membrane, cytosolic, and post-nuclear fractions...

there has been considerable inconsistency regarding the potential relationship between dyslipidemia and bone metabolism. the inflammatory stimulation through the receptor activator of the nuclear factor kappa-b ligand (rankl)/ receptor activator of the nuclear factor kappa-b (rank)/ osteoprotegerin (opg) pathway could be the infrastructural mechanism for hypercholesterolemia-induced bone loss. ...