The aim of this study was to develop an efficient enzymatic glycerolysis system together with physical fractionation operation for the production of monoacylglycerols (MAG) containing polyunsaturated fatty acids (PUFA), especially, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) from tuna oil. The enzymatic glycerolysis system at 40-70 °C is unfortunately a multiple-phase system, whi...

In order to obtain phosphatidylcholine (PC) with higher amount of oleic acid, the interesterification between soybean PC and Camellia oleifera oil (COO) rich in oleic acid catalyzed by lipase was studied in hexane. For this aim three commercially available immobilized lipases (Novozym 435, Lipozyme TLIM and Lipozyme RMIM) were assayed and Novozym 435 was finally selected for further optimizatio...

Background: Enzymatic kinetic resolution is proved as an efficient strategy of accessing chiral secondary alcohols in organic synthesis. Although several synthetic methods have been developed for the preparation of chiral acetylenic alcohol, biotransformation remains as a direct approach in the synthesis of polyacetylene lipids, which represent an intriguing class of marine natural products fea...

Fusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping-pong bi-bi mechanism (inhibition phenomena taken into account) was applied as a complex kinetic model. The parameters of the mo...

With the ever-increasing demand for clean technology in industrial sector, natural methods, such as enzyme-catalyzed, represent a sustainable alternative to chemical processes. In this context, synthesis of benzyl benzoate ester using commercial immobilized lipases was evaluated. For this, kinetic study carried out determine reaction time (24 h) and enzyme concentration (10 wt%). Then, 22 full ...

The lipase B from Candida antarctica (Novozym 435®, CALB) efficiently catalyzed the kinetic resolution of some aliphatic secondary alcohols: (±)-4-methylpentan-2-ol, (±)-5-methylhexan-2-ol, (±)-octan-2-ol, (±)-heptan-3-ol and (±)-oct-1-en-3-ol. The lipase showed excellent enantioselectivities in the transesterifications of racemic aliphatic secondary alcohols producing the enantiopure alcohols ...

We report the use of an immobilised form of Candida antarctica lipase B, Novozym((R)) 435, in a preliminary investigation into the development of a continuous flow reactor capable of performing the chemo-enzymatic oxidation of alkenes in high yield and purity, utilising the commercially available oxidant hydrogen peroxide (100 volumes). Initial investigations focussed on the lipase-mediated oxi...

This work was focused on the enzymatic esterification of glycerol and ibuprofen at high concentrations in two triphasic systems composed toluene+ibuprofene (apolar) or glycerol–water (polar) liquid phases, a solid phase with industrial immobilized lipase B from Candida antarctica named Novozym®435 (N435) acting as biocatalyst. Based preliminary study, concentration enzyme set 30 g·L−1 stirring ...