timur began his domination by the help he received from parts of the chaghataid troops. then and concurrent to extending his realm and power, he recruited defeated populations as new part of his army. simultaneously he established household – provincial powers in his occupations which his sons and grandsons ruled over them. this political – administrative tradition was followed by his successor...

davood became fearful when he encountered with his diabetes diagnosis for first time. what effect does this disease can have on davood ’ s lifestyle? in this case, the nurse describes how davood ’ s fear is released by education. i met davood in 1992 for first time. he had been admitted in ward since two days ago, and he had been forced to stay at the hospital through neu-rooz holidays. he acce...

Bending of purple membranes in dependence on the pH analyzed by AFM and single molecule force spectroscopy R.-P. Baumann, M. Schranz and N. Hampp, Phys. Chem. Chem. Phys., 2010 DOI: 10.1039/b919729j Bifunctional polyacrylamide based polymers for the specific binding of hexahistidine tagged proteins on gold surfaces Lucas B. Thompson, Nathan H. Mack and Ralph G. Nuzzo, Phys. Chem. Chem. Phys., 2...

We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically enc...

BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...