Introduction In this study, we validated the changes in microRNA (miRNA) expression hypoxic human kidney 2 (HK2) cell-derived extracellular vesicles (EVs). Additionally, investigated mechanism by which miRNA that are derived from EVs alleviated renal ischemia-reperfusion (I/R) injury. Material and methods HK2 cells were treated a chamber (1% O2) for 12 h.EVs obtained as supernatant ultracentrif...

INTRODUCTION It has been speculated that short (-1-h) exposures to intermittent normobaric hypoxia at rest can enhance subsequent exercise performance. Thus, the present study investigated the effect of daily resting intermittent hypoxic exposures (IHE) on peak aerobic capacity and performance under both normoxic and hypoxic conditions. METHODS Eighteen subjects were equally assigned to eithe...

Cardiosphere-derived cells (CDCs) have emerged as one of the most promising stem cell types for cardiac protection and repair. Exosomes are required for the regenerative effects of human CDCs and mimic the cardioprotective benefits of CDCs such as anti-apoptotic effect in animal myocardial infarction (MI) models. Here we aimed to investigate the anti-apoptotic effect of the hypoxic and normoxic...

Background: Alveolar hypoxia induces monophasic pulmonary vasoconstriction in vivo, biphasic vasoconstriction in the isolated pulmonary artery, and controversial responses in the isolated perfused lung. Pulmonary vascular responses to sustained alveolar hypoxia have not been addressed in the isolated perfused rat lung. In this study, we investigated the effect of sustained hypoxic ventilation o...

The apoptotic effects of maslinic acid (MA) at 4, 8, 16, 32 and 64 μmol/L on human lung cancer A549 cells under normoxic and hypoxic conditions were examined. MA at 4-64 and 16-64 μmol/L lowered Bcl-2 expression under normoxic and hypoxic conditions, respectively (p < 0.05). This agent at 4-64 μmol/L decreased Na+-K+-ATPase activity and increased caspase-3 expression under normoxic conditions, ...

Resuscitation and prolonged ventilation using 100% oxygen after cardiac arrest is standard clinical practice despite evidence from animal models indicating that neurologic outcome is improved using normoxic compared with hyperoxic resuscitation. This study tested the hypothesis that normoxic ventilation during the first hour after cardiac arrest in dogs protects against prelethal oxidative stre...

Although many lung diseases are associated with hypoxia, alveolar type II epithelial (ATII) cell impairment, and pulmonary surfactant dysfunction, the effects of O(2) limitation on metabolic pathways necessary to maintain cellular energy in ATII cells have not been studied extensively. This report presents results of targeted assays aimed at identifying specific metabolic processes that contrib...