Atalantia monophylla (L.) DC. is a medium sized medicinal tree used in Ayurveda, Siddha and Traditional systems of medicine. It is used for the treatment of chronic rheumatism and paralysis, as an antidote for snake bites and shows antibacterial, antifungal and antifeedant activity. The study aims to develop a protocol for multiple shoot induction from nodal and root explants. After surface ste...

Recent experiments have suggested a pathway of genes that regulate left-right asymmetry in vertebrate embryogenesis. The most downstream member of this cascade is nodal (XNR-1 in frogs), which is expressed in the left-side lateral mesoderm. Previous work in the chick [Levin, 1998] suggests that an inductive interaction by Shh (Sonic hedgehog) present at the midline was needed for the left-sided...

In the present investigation, a micropropagation protocol has been developed for Vanilla borneensis Rolfe – critically endangered orchid through multiple shoot regeneration. Through in vitro regeneration from both nodal and tip explants, maximum (100%) induction was observed. The minimum time required bud observed (5–7 days) on medium supplemented with BAP (4.44 mM) + KIN (2.32 as compared to e...

In the present study, we have established a stable transformation protocol via Agrobacterium tumafacines for the pharmaceutically important Withania somnifera. Six day-old nodal explants were used for 3 day co-cultivation with Agrobacterium tumefaciens strain LBA4404 harbouring the vector pCAMIBA2301. Among the different injury treatments, sonication, vacuum infiltration and their combination t...

An efficient in vitro propagation system for an elite clone EC-353508 of Artemisia annua L., an important antimalarial drug plant has been developed. The best organogenic response, including adventitious multiple shoots and their elongation, was obtained when nodal explants were cultured on MS medium supplemented with 10μM 2-isopentenyl adenine (2iP). An average of 17.7±0.88 shoots with an aver...

Grape is important crop with high nutritional value, however there is a great need for improving the quality of the crop. Cultures were maintained in vitro on MS medium supplemented with 2.5 mg/L BAP+ TDZ 3.0 mg/ L. The effect of different doses of gamma irradiation as well as chemical mutagenesis by Sodium azide was studied. It was noted that doses up to 5 Gy helped to increase plant height fr...