نتایج جستجو برای: nitrocellulose

تعداد نتایج: 1717  

Journal: :Combustion and Flame 2021

Safety improvements in the field of energetic materials led to development insensitive gun propellants. This article deals with ignition and combustion parameters two commercial propellants, respectively based on RDX nitrocellulose. Ignition is obtained thanks a laser diode probabilities are given, along delays, overpressures propagation rates. Experiments performed under nitrogen argon atmosph...

2017
Meilin Zhu Uwa Obahiagbon

Point-of-care (POC) diagnostics tools provide lowcost methods of disease detection in low resource settings. Our current platform detects antibodies in serum to recombinant protein biomarkers on glass substrate. Most commercial POC tests utilize nitrocellulose lateral flow format with colorimetric detection. Nitrocellulose enables fluid handling, serum filtration, better protein immobilization,...

Journal: :Indian journal of biochemistry & biophysics 2002
Hina Jamil Mohammed Saleemuddin

A simple strategy to remarkably increase the sensitivity of detection of antigens applied as dot or western blot on nitrocellulose membrane using human serum albumin as model antigen has been described. This involves subjecting the antigen bearing nitrocellulose strips to multiple incubation cycles with primary antibody and enzyme conjugated secondary antibody prior to staining for enzyme activ...

A. Moghimi B. Naderizadeh, M. Shahi

Electrospinning was applied to nitrocellulose (NC) solutions  in DMF, DMSO  and  Ethyl  methyl  keton  to  obtain  NC  nanofibers. Electrospraying  process  was  observed  when  DMF  was  used  as solvent.  In  comparison  to NC/DMF, NC/DMSO  solution  resulted  in the  formation  of  a  coating  film  as  indicate  by  SEM.  Two  different electrospun  nanofibers,  smooth  nanofibers  wit...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1993
I Wong T M Lohman

Nitrocellulose-filter binding is a powerful technique commonly used to study protein-nucleic acid interactions; however, its utility in quantitative studies is often compromised by its lack of precision. To improve precision and accuracy, we have introduced two modifications to the traditional technique: the use of a 96-well dot-blot apparatus and the addition of a DEAE membrane beneath the nit...

Journal: :Nucleic acids research 1977
E Spicer J Schwarzbauer G R Craven

E. coli ribosomal proteins are retained by nitrocellulose filters. In contrast, 16S RNA passes through nitrocellulose filters. We have found that specific protein-RNA complexes involving single proteins also pass through nitrocellulose filters. Thus, by utilizing radioactively labeled r-proteins, nitrocellulose filtration can be used to study directly and sensitively the stoichiometry of r-prot...

Journal: :BioTechniques 2000
F B Hashemi G T Spear L Madsen J Mollenhauer

We previously reported that cervicovaginal lavages (CVL) contain a factor that enhances the replication of human immunodeficiency virus (HIV) by increasing virus transcription in T cells and monocytoid cells. This factor was named the HIV-inducing factor (HIF). To determine the molecular mass of HIF, we adapted a blot technique that involves nonreducing SDS-PAGE of CVL samples and electrophoret...

Journal: :Zeitschrift für analytische Chemie 1893

Journal: :The Journal of Cell Biology 1985
D J Donaldson J T Mahan D L Hasty J B McCarthy L T Furcht

The interaction of migrating newt epidermal cells with the extracellular matrix protein, fibronectin, was studied. Pieces of nitrocellulose coated with intact human plasma fibronectin or proteolytically derived fragments were implanted into wounded limbs so that the coated nitrocellulose served as wound bed for migrating epidermal cells as they attempted to form a wound epithelium. Epidermal ce...

Journal: :Journal of clinical microbiology 1984
L Beutin L Bode T Richter G Peltre R Stephan

A modification of the enzyme-linked immunosorbent assay for a sensitive and rapid visual detection of heat-labile enterotoxins from Escherichia coli and Vibrio cholerae is described. Small amounts of bacterial supernatant fluids are bound to nitrocellulose filters which are used as sorbents in the nitrocellulose enzyme-linked immunosorbent assay. The test is based on the immunological similarit...

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