نتایج جستجو برای: nitrocellulose
تعداد نتایج: 1717 فیلتر نتایج به سال:
Safety improvements in the field of energetic materials led to development insensitive gun propellants. This article deals with ignition and combustion parameters two commercial propellants, respectively based on RDX nitrocellulose. Ignition is obtained thanks a laser diode probabilities are given, along delays, overpressures propagation rates. Experiments performed under nitrogen argon atmosph...
Point-of-care (POC) diagnostics tools provide lowcost methods of disease detection in low resource settings. Our current platform detects antibodies in serum to recombinant protein biomarkers on glass substrate. Most commercial POC tests utilize nitrocellulose lateral flow format with colorimetric detection. Nitrocellulose enables fluid handling, serum filtration, better protein immobilization,...
A simple strategy to remarkably increase the sensitivity of detection of antigens applied as dot or western blot on nitrocellulose membrane using human serum albumin as model antigen has been described. This involves subjecting the antigen bearing nitrocellulose strips to multiple incubation cycles with primary antibody and enzyme conjugated secondary antibody prior to staining for enzyme activ...
Electrospinning was applied to nitrocellulose (NC) solutions in DMF, DMSO and Ethyl methyl keton to obtain NC nanofibers. Electrospraying process was observed when DMF was used as solvent. In comparison to NC/DMF, NC/DMSO solution resulted in the formation of a coating film as indicate by SEM. Two different electrospun nanofibers, smooth nanofibers wit...
Nitrocellulose-filter binding is a powerful technique commonly used to study protein-nucleic acid interactions; however, its utility in quantitative studies is often compromised by its lack of precision. To improve precision and accuracy, we have introduced two modifications to the traditional technique: the use of a 96-well dot-blot apparatus and the addition of a DEAE membrane beneath the nit...
E. coli ribosomal proteins are retained by nitrocellulose filters. In contrast, 16S RNA passes through nitrocellulose filters. We have found that specific protein-RNA complexes involving single proteins also pass through nitrocellulose filters. Thus, by utilizing radioactively labeled r-proteins, nitrocellulose filtration can be used to study directly and sensitively the stoichiometry of r-prot...
We previously reported that cervicovaginal lavages (CVL) contain a factor that enhances the replication of human immunodeficiency virus (HIV) by increasing virus transcription in T cells and monocytoid cells. This factor was named the HIV-inducing factor (HIF). To determine the molecular mass of HIF, we adapted a blot technique that involves nonreducing SDS-PAGE of CVL samples and electrophoret...
The interaction of migrating newt epidermal cells with the extracellular matrix protein, fibronectin, was studied. Pieces of nitrocellulose coated with intact human plasma fibronectin or proteolytically derived fragments were implanted into wounded limbs so that the coated nitrocellulose served as wound bed for migrating epidermal cells as they attempted to form a wound epithelium. Epidermal ce...
A modification of the enzyme-linked immunosorbent assay for a sensitive and rapid visual detection of heat-labile enterotoxins from Escherichia coli and Vibrio cholerae is described. Small amounts of bacterial supernatant fluids are bound to nitrocellulose filters which are used as sorbents in the nitrocellulose enzyme-linked immunosorbent assay. The test is based on the immunological similarit...
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