A simple method for detection and enumeration of alkylbenzenesulfonate (ABS)-degrading microorganisms by using agar plates was developed and used in microbiological studies of coastal marine and polluted river waters. The method depends upon the color responses of neutral red in alkaline medium. Neutral red changes from pink, when it enters into ABS micelles, to yellow, when the ABS is degraded...

Numerous natural compounds have a potential for therapeutic applications, but may have to be chemically modified to alter toxic side effects. We investigated structural parameters that could affect the cytotoxicity of isocoumarins similar to 9,10-dihydroxy-5,7-dimethoxy-1H-naphtho(2,3c)pyran-1-one (paepalantine 1). Paepalantine 1 has antimicrobial activity, as well as significant in vitro cytot...

I investigations carried out in this laboratory have been concerned with the initial responses of the corneal stromal cells to injury. One reaction which occurs in the stromal cells within a few hours after injury to the cornea is the activation of the fibrocytes to take up the vital dye, neutral red.' This reaction does not occur in the normal corneal stromal cells of the rat, but cells activa...

After treatment with neutral red at low concentrations (10(-6), 10(-5) M), the chromatin of chicken erythrocytes shows an intensive red fluorescence, which is reduced or practically abolished when nuclei become stained by using the dye at concentrations higher than 10(-4) M. Both the fluorescence and staining reactions are dependent on the DNA content of chromatin. Neutral red fluorescence of n...

Neutral red staining was evaluated as an acute outcome assessment method in rat models of cerebral ischemia by comparison with histological infarction volume. Fischer 344 rats (n = 48) were used in three different models of middle cerebral artery (MCA) occlusion: proximal MCA occlusion (n = 16), distal MCA occlusion followed by ipsilateral common carotid artery (CCA) occlusion (distal MCA/CCA o...

The arousal theory of color proposes that red is associated with arousal. Research on the color-in-context theory, in turn, states that the context in which red is perceived influences its valence-related meaning and behavioral responses to it. This study faces and integrates these theories by examining the influence of red on both arousal and valence perceptions of test-relevant and neutral st...

Quantitative light absorption data of the neutral free flavosemiquinone are reported. It is shown that the visible range of the spectrum of the radical is drastically dependent on the solvent. The spectra of the neutral blue flavosemiquinone are compared with those of the blue flavoprotein radicals and conclusions are drawn concerning the environment of the protein-bound flavosemiquinone. The s...

A clinical trial was undertaken to compare the efficacy of neutral red photodynamic inactivation treatment of genital herpes infections with that of a non-photoactive dye, phenol red, as a control. In a series of nineteen patients with virologically proven herpes genitalis who were adequately followed, eleven were treated with neutral red, and eight with phenol red; no difference in response to...

as a measure of gastric function, and various standardized tests have been devised. Owing, however, to the wide range of acid secretion in normal people, to the inconstant results obtained by repeating a test on many individuals, to the frequent occurrence of results well within the normal range in patients suffering from gastritis, gastric carcinoma, or peptic ulcer, and to the fact that from ...