Hen egg lysozyme-carboxymethyl dextran (HEL-CMD) conjugate was prepared by using water-soluble carbodiimide as a model protein-acidic polysaccharide conjugate for improving the protein function. An acid-amide bond between HEL and CMD was confirmed by SDS-PAGE, isoelectric focusing and IR spectra. The molar ratio of CMD to HEL in the conjugate was 1:1. The isoelectric point of the conjugate was ...

Conformational features of reduced and disulfide intact hen egg white lysozyme in aqueous 1,4-dioxane and 3-chloro-1, 2-propanediol solutions have been examined using circular dichroism and fluorescence spectroscopy. We find that in presence of 1, 4-dioxane, reduced lysozyme assumes a relatively compact conformational form with secondary structure closer to native state and no tertiary structur...

A Monte Carlo algorithm that searches for the optimal docking configuration of hen egg white lysozyme to an antibody is developed. Both the lysozyme and the antibody are kept rigid. Unlike the work of other authors, our algorithm does not attempt to explicitly maximize surface contact, but minimizes the energy computed using coarse-grained pair potentials. The final refinement of our best solut...

Proton transfer reactivity of isolated charge states of the protein hen egg-white lysozyme shows that multiple distinct conformations of this protein are stable in the gas phase. The reactivities of the 9+ and 10+ charge state ions, formed by electrospray ionization of "native" (disulfide-intact) and "denatured" (disulfide-reduced) solutions, are consistent with values calculated for ions in th...

Kabool Sheikh, Muhamadan, aged 20, was admitted into the Gay a Pilgrim Hospital on the 21st February at 10 a.m , and examined by me at 11 a.m. She was a primipara whose last menstruation was eight months ago, and who had been suffering from continuous pain in the abdomen for the last ten days. Previous history.? She is said to have been quite well w hen suddenly a severe continuous pain of a pi...

Molecular dynamics simulations of four peptides taken from the hen lysozyme sequence have been used to generate models for non-native protein conformations. Comparisons between the different peptides and with experimental data for denatured lysozyme and peptide fragments provides insight into the characteristics of the conformational ensembles populated in these non-native states and the depend...

BACKGROUND Sphingosine-1 receptor 1 (S1P1) plays a major role in regulating lymphocyte egress from peripheral lymph tissue. Lymphocyte trafficking is potentially a critical response to tumors and to tumor vaccines. Also, the receptor has been shown to influence metastasis. However, there is little information on its expression in the aged ovary or ovarian tumors. As a basis for further studies ...

this study was carried out to compare the histomorphological structures of oviductal regionsof the apparently healthy adult laying hens (gallus gallus dometicus) and ducks (ansa ansadomesticus). for this purpose, 20 hens and 20 female ducks aged between 1–1.5 years were used.after euthanasia, the oviducts were dissected out and all of the gross morphometrical parametersincluding length, width a...

In this work, an alternative procedure to retrofit an industrial plant is proposed, based on a new heat exchanger network (HEN) synthesis and evolution algorithm. The idea is to consider the retrofitting as a grassroots design, but with match constrains accounted for during the synthesis. First, a lower bound for the HEN total annual cost (TAC) is determined and a new HEN is synthesized. A comp...

abbasali gheisari*, peyman ghayor department of animal science, isfahan research center for agriculture and natural resources, isfahan,iran   * corresponding author e-mail: [email protected]     received: 15 august 2013 accepted: 21 november 2013       abstract   this experiment was conducted to investigate the effect of feeding 5 experimental diets containing 0, 5, 10, 15 and 20% rapesee...