Significant recent advances have been made in studies of the major dissimilatory nitrate reductase (NarGHI) of Escherichia coli. This enzyme is a complex iron-sulfur ([Fe-S]) molybdoenzyme that oxidizes menaquinol or ubiquinol at a periplasmically oriented Q-site (Qp site), and reduces nitrate at a cytoplasmically-oriented molybdo-(bismolybdopterin guanine dinucleotide) (Mo-bisMGD) cofactor. Th...

RNA was isolated from cultures of Escherichia coli strain MG1655 and derivatives defective in fnr, narXL, or narXL with narP, during aerobic growth, or anaerobic growth in the presence or absence of nitrate or nitrite, in non-repressing media in which both strain MG1655 and an fnr deletion mutant grew at similar rates. Glycerol was used as the non-repressing carbon source and both trimethylamin...

For the human health, Mycobacterium tuberculosis (MTB) is the deadliest enemy since decades due to its multidrug resistant strains. During latent stage of tuberculosis infection, MTB consumes nitrate as the alternate mechanism of respiration in the absence of oxygen, thus increasing its survival and virulence. NarL is a nitrate/nitrite response transcriptional regulatory protein of two-componen...

The majority of bacterial promoters are regulated by at least two transcription factors. Many of these promoters are regulated by two activators, with expression being co-dependent on the activity of both activators [l] . At most of these promoters, one of the activators responds to a ‘global’ regulatory signal whilst the other responds to a ‘specific’ signal. For example, transcription initiat...

This paper describes a LIDAR for atmospheric measurement and probing (LAMP). The LAMP system is a commercial LIDAR unit that uses the design of boundary layer LIDAR (BLL), an optical radar technology developed at the National Atmospheric Research Laboratory (NARL) for atmospheric applications. The LAMP technology is useful for remote monitoring of atmospheric particulate and clouds in the tropo...

BACKGROUND In 2009, there were 8273 local screening laboratories, 254 confirmatory laboratories, 35 provincial confirmatory central laboratories and 1 National AIDS Reference Laboratory (NARL) in China. These laboratories were located in Center for Disease Control and Prevention (CDC) facilities, hospitals, blood donation clinics, maternal and child health (MCH) hospitals and border health quar...

HCP (hybrid-cluster protein) contains two Fe/S clusters, one of which is a hybrid [4Fe-2S-2O] cluster. Despite intensive study, its physiological function has not been reported. The Escherichia coli hcp gene is located in a two-gene operon with hcr, which encodes an NADH-dependent HCP reductase. E. coli HCP is detected after anaerobic growth with nitrate or nitrite: possible roles for it in hyd...

background: the variation of the radon progeny concentration in outdoor environment and meteorological parameters at fine resolution were studied for one year at a continental location, national atmospheric research laboratory, gadanki, india.  materials and methods: the concentrations were measured using alpha progeny meter by collecting air samples at a height of 1 m above the earth’s surface...

Two-component systems, sensor kinase-response regulator pairs, dominate bacterial signal transduction. Regulation is exerted by phosphorylation of an Asp in receiver domains of response regulators. Lability of the acyl phosphate linkage has limited structure determination for the active, phosphorylated forms of receiver domains. As assessed by both functional and structural criteria, berylloflu...

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