Semen was diluted 1:9 with egg yolk-citrate medium containing 0.31--3.1 M (final concentration) formamide, butyramide, acetamide, propionamide, dimethylformamide, lactamide, malomide, ethylene glycol, trimethylene glycol, dimethylsulphoxide (DMSO) or glycerol. After 30 min incubation at 20 degrees C, sperm motility was superior in hypertonic solutions of acetamide, lactamide, dimethylsulphoxid...

The yeast Kluyveromyces lactis has been extensively used as a host for heterologous protein expression. A necessary step in the construction of a stable expression strain is the introduction of an integrative expression vector into K. lactis cells, followed by selection of transformed strains using either medium containing antibiotic (e.g., G418) or nitrogen-free medium containing acetamide. In...

In previous communications from this laboratory we have reported that the hydroxylation of the carcinogen N-(2-fluorenyl)acetamide can be readily demonstrated in slices (1, 2) and in homogenates fortified with diphosphopyridine nucleotide and succinate (3), but not in unfortified homogenates (1, 3). It was also found that the binding of the radioactivity of N-(Zfluorenyl) acetamide-g-C*4 to cel...

The rabbit is considered to be a valuable laboratory animal. We compared glycerol, lactamide, acetamide, and dimethylsulfoxide (DMSO) as cryoprotectants in egg-yolk diluent of ejaculated Japanese white rabbit spermatozoa for improvement of sperm cryopreservation methods. Rabbit semen was frozen with 1.0 M glycerol, lactamide, acetamide, or DMSO in plastic straws. Forward progressive motility an...

To elucidate the influence of amino (-NH2) and acetamide (-NHCOCH3, -NAc) groups in sugar chains on their ionization and fragmentation, cycloamyloses (cyclodextrins, CyDs) and lacto-oligosaccharide are analyzed by MALDI TOF/TOF and ESI Q-TOF mass spectrometry. CyD derivatives substituted by amino or acetamide groups are ideal analytes to extract the function group effects, which are amino-CyD w...

Novel genes that function in the conversion of indole-3-acetamide (IAM) into indole-3-acetic acid (IAA), which were previously thought to exist only in the bacterial genome, have been isolated from plants. The finding of the AtAMI1 gene in Arabidopsis thaliana and the NtAMI1 gene in Nicotiana tabacum, which encode indole-3-acetamide hydrolase, indicates the existence of a new pathway for auxin ...

The conformation of the N-H bond in the structure of the title compound, C(9)H(10)ClNO, is syn to the meta-methyl group, in contrast to the anti conformation observed with respect to the meta-nitro group in 2-chloro-N-(3-nitro-phen-yl)-acetamide. The asymmetric unit of the title compound contains two mol-ecules. The geometric parameters of the title compound are similar to those of 2-chloro-N-(...

The conformation of the N-H bond in the title compound (34DMPA), C(10)H(13)NO, is syn to the 3-methyl substituent in the aromatic ring, in contrast to the anti conformation observed with respect to the 3-chloro substituent in N-(3,4-dichloro-phen-yl)acetamide (34DCPA). The asymmetric unit of the structure contains three mol-ecules. The bond parameters in 34DMPA are similar to those in 34DCPA, N...

Naphthalene is an ubiquitous pollutant of the environment and the biodegradation of this pollutant has been receiving constant scientific consideration. The aim of this study was to isolate and identify bacteria that could degrade naphthalene from three regions of the Gol Gohar Mine at Sirjan, Iran. In this study, the total naphthalene degrading bacteria were quantified with the most probable n...

in the study of the effects of salicylate on population density of naphthalene degrading bacteria and heterotrophic bacteria in a sample or: soil polluted with aromatic hydrocarbons, two different concentrations of salicylate (16, 160) ?g / gr of soil incubated for 30 days. at 160 ?g / gr, salicylate enhanced the density of naphthalene degraders and heterotrophic bacteria for 30 days. no chenge...