(1) The marine teleost fish, Lagodon rhomboides, can only tolerate fresh water (5 mM Na) if Ca is also present (10 mM). Transfer to Ca-free fresh water is followed by a substantial increase in radioactive Na efflux with little or no change in the transepithelial potential. Addition of the chelating agent EDTA (2 mM) further increases Na efflux. Fish left in Ca-free fresh water for 2-5 h die wit...

Manual procedures suitable for use on standard benchtop spectrophotometers have been developed for the enzymatic determination of Na+ and K+ in serum. Both assays require only minimal modification of reagents already available for BM/Hitachi analyzers and are performed in an endpoint mode, allowing up to 20 assays per run. The addition of a stop reagent is required--dipotassium EDTA for the Na+...

The effects of acute i.v. inorganic phosphate (Pi) loading were studied on nembutalized mongrel dogs previously subjected to unilateral splanchnicotomy ("renal denervation"). GFR (51Cr-EDTA) was not different on the intact and the denervated side, while urine output (V), sodium excretion (UNaV), and urinary excretion (UPiV) of inorganic phosphate of denervated kidneys were significantly increas...

Foi estabelecido um método cromatográfico rápido e simples para a determinação de riboflavina em leite bovino, na presença de tetraciclinas. Para tanto, as proteínas foram precipitadas com ácido tricloroacético e a separação realizada por CLAE em fase reversa com detector de fluorescência. Foi utilizada uma coluna C 8 com eluição isocrática e fase móvel acetato de sódio 0,1 mol L, cloreto de cá...

BACKGROUND Measurements of 24-h fractionated urinary metanephrines and catecholamines are used for the diagnosis of pheochromocytoma, but adequate information is needed regarding collection, storage, and shipment conditions. METHODS Spot urine samples were collected from 8 healthy volunteers. Aliquots were immediately frozen at -20 degrees C, or acidified to pH 4 and then frozen either direct...

Fusarium oxysporum, isolated from infected tomato plant parts, produced maximum cellulase at optimum parameters (pH, 6.0; temp., 50°C; and incubation period, 12 d) in cellulase enzyme production broth having 1% CMC (carboxy methyl cellulose) as a cellulose substrate. Activities of purified cellulases (mol wt, 24, 29 and 45 kDa) were stimulated by concentrations (0-70 mM) of Na and Mg, while EDT...

Ethylenediaminetetraacetate added to washed cells in tris (hydroxymethyl) aminomethane buffer increases the surface permeability of all coliforms tested, including nine strains of Escherichia coli, one strain of Aerobacter aerogenes, and four species of Salmonella. Although this treatment increases passive permeability, two transport systems tested were unaffected. The permeability change is co...

In this study, we improved the eggshell-membrane separation process by separating the shell and membrane with EDTA solution, evaluating effects of three different extraction solutions (acetic acid, EDTA, and phosphate solution), and co-purifying multiple eggshell proteins with two successive ion-exchange chromatography procedures (CM Sepharose Fast Flow and DEAE Sepharose Fast Flow). The recove...