As the most nucleophilic site in DNA, the guanine N7 atom is a major site of adduction by a large number of alkylating mutagens and carcinogens. Aflatoxin B1, a powerful mutagen, is believed to act through its reaction with this DNA site. On the basis of the specificity of base substitutions induced by various adduct forms of aflatoxin, we have proposed that bulky guanine N7 adducts elicit base...

Electrical signals elicited by integrin interaction with ECM components and their role in neurite outgrowth were studied in two clones (N1 and N7) isolated from 41A3 murine neuroblastoma cell line. Although the two clones similarly adhered to fibronectin (FN) and vitronectin (VN), this adhesion induced neurite outgrowth in N1 but not in N7 cells. Patch clamp recordings in whole cell configurati...

UNLABELLED HIV-1 establishes persistent infection in part due to its ability to evade host immune responses. Occlusion by glycans contributes to masking conserved sites that are targets for some broadly neutralizing antibodies (bNAbs). Previous work has shown that removal of a highly conserved potential N-linked glycan (PNLG) site at amino acid residue 197 (N7) on the surface antigen gp120 of H...

We conducted a repeated-measures cohort study of coke oven workers to evaluate the relationships between the traditional exposure biomarker, urinary 1-hydroxypyrene (1-OHP), and a series of biomarkers, including urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), N7-methylguanine (N7-MeG), acute toxicity, and mutagenicity. A total of eight spot urine samples were collected from each high-exp...

We show that the maximum number of occurrences of a given angle in a set of n points in R3 is O(n7/3), and that a right angle can actually occur Ω(n7/3) times. We then show that the maximum number of occurrences of any angle different from π/2 in a set of n points in R4 is O(n5/2β(n)), where β(n) = 2O(α(n) 2) and α(n) is the inverse Ackermann function.

The N7 of purine nucleotides presents one of the most dominant metal ion binding sites in nucleic acids. However, the interactions between kinetically labile metal ions like Mg(2+) and these nitrogen atoms are inherently difficult to observe in large RNAs. Rather than using the insensitive direct (15)N detection, here we have used (2)J-[(1)H,(15)N]-HSQC (Heteronuclear Single Quantum Coherence) ...

First we will prove a small lemma. Lemma 1. Let P be a p-Sylow subgroup of G. Let N be a normal subgroup of G such that P ⊂ N . Then all p-Sylow subgroups are in N . Proof. Let P̃ be a p-Sylow subgroup that is not P . Then by the Sylow Theorems, we know that gPg−1 = P̃ for some g ∈ G. Since P ⊂ N , and N is normal, we know that gPg−1 ⊂ N and hence P̃ ⊂ N . Thus all p-Sylow subgroups are in N . Now...

The major DNA adduct formed from the carcinogen ethylene dibromide (1,2-dibromoethane, EDB) is S-[2-(N7-guanyl)ethyl]glutathione, resulting from the reaction of guanyl residues with the half-mustard S-(2-bromoethyl)glutathione, which is generated by glutathione S-transferase-catalyzed conjugation of EDB with glutathione. The half-life of the alkylating species [putative S-(2-bromoethyl)glutathi...

The kinetics of the reactions of 5'-AMP and 5'-GMP with excess cis-[Pt(NH,),CI,] in the presence of KCI at pH* 6.5 have been investigated by 'H NMR and analyzed by nonlinear numerical integration and optimization techniques. The species formed have been further characterized by 195Pt NMR. The reaction with 5'-AMP involves a branching pathway: 5'-AMP reacts with ~ i s [ P t ( N H ~ ) ~ c l , ] t...