We have developed a N6-dimethylallyladenine (cytokinin) dehydrogenase-based microbiosensor for real-time determination of the family of hormones known as cytokinins. Cytokinin dehydrogenase from Zea mays (ZmCKX1) was immobilised concurrently with electrodeposition of a silica gel film on the surface of a Pt microelectrode, which was further functionalized by free electron mediator 2,6-dichlorop...

BACKGROUND Omega6 (n6) polyunsaturated fatty acids (PUFAs) and their metabolites are involved in cell signaling, inflammation, clot formation, and other crucial biological processes. Genetic components, such as variants of fatty acid desaturase (FADS) genes, determine the composition of n6 PUFAs. METHODS AND RESULTS To elucidate undiscovered biological pathways that may influence n6 PUFA comp...

The density of adenosine receptors in membranes derived from rat hearts in 25 times lower than the density of receptors in rat brain membranes. Consequently, adenosine radioligands which are useful in brain such as l-[3H]phenylisopropyladenosine, [3H]cyclohexyladenosine, [3H]-2-chloroadenosine and l-[125I]hydroxyphenylisopropyladenosine are of limited usefulness in heart, due to a high ratio of...

We present combined BIMA interferometer and IRAM 30 m Telescope data of N2H + 1–0 line emission across the nearby dense, star forming core Ophiuchus A (Oph A) at high linear resolution (e.g., ∼1000 AU). Six maxima of integrated line intensity are detected which we designate Oph A-N1 through N6. The N4 and N5 maxima are coincident with the starless continuum objects SM1 and SM2 respectively but ...

Acyclic nucleoside phosphonates (ANPs) are potent antiviral agents effective against replication of DNA viruses and retroviruses including human immunodeficiency virus (HIV). Prototype compound 9-(R)-[2-(phosphonomethoxy)propyl]adenine (tenofovir) is a principal component of drugs widely used in the treatment of HIV infection (Viread, Truvada). Besides their antimetabolic mode of action, ANPs p...

High hydrostatic pressure affects in various ways the reactivity and the stability of biogenic macromolecules [ 11. Pressures of several hundred bars reduce or increase enzymic activity [2-41. Proteins are usually irreversibly denaturated by pressures in excess of 1 kbar. On the other hand, calf thymus DNA is stabilized by increased pressure [5]. In general, an increase in pressure shifts an eq...

BACKGROUND Infant temperament predicts a range of developmental and behavioral outcomes throughout childhood. Both maternal fatty acid intake and psychosocial stress exposures during pregnancy may influence infant temperament. Furthermore, maternal race may modify prenatal diet and stress effects. The goals of this study are to examine the joint effects of prenatal diet and stress and the modif...

the lowest lipid levels of capoeta erhani observed in winter and vice versa in summer. the fatty acid composition of the fillets was significantly different among seasons ( p <0.05), while the variations among sexes were not at the same degrees ( p >0.05). the ratios of the unsaturated fatty acids (ufas) were higher than half of the total fatty acids among all seasons. the level of pufa was hig...

Synthesis of guanosine 3'-phosphodiesters of 1, N6-ethenoadenosine-2-sulfonate or 1, N6-etheno-2-azaadenosine can be done readily with ribonuclease N1. There is a 10-fold difference in quantum yield between the phosphodiesters and the starting fluorescent nucleosides. This difference provides the basis for the development of a fluorescent assay for nuclease.