1. Nicholas R, Churchward C. Contagious caprine pleuropneumonia: new aspects of an old disease. Transbound Emerg Dis. 2012;59:189–96. http://dx.doi. org/10.1111/j.1865-1682.2011.01262.x 2. Fischer A, Shapiro B, Muriuki C, Heller M, Schnee C, Bongcam-Rudloff E, et al. The origin of the ‘Mycoplasma mycoides cluster’ coincides with domestication of ruminants. PLoS ONE. 2012;7:e36150. h t tp : / /d...

were conducted by using MEGA version 4.1 (www.megasoftware. net). The partial lppA gene nucleotide sequences of isolates GN407 and GN408 were submitted to GenBank under accession nos. HQ699892 and HQ699893, respectively. PCR amplifi cations of the 2 joint fl uids and their cultures were positive for M. leachii. When we compared the complete 16S rRNA gene and the partial lppA gene, the 2 isolate...

DNA amplification techniques offer considerable promise for the identification of Mycoplasma mycoides cluster members. They avoid antigenic cross-reactivity and variability that hamper serological methods. Many sets of primers, specific of these different members and of Mycoplasma putrefaciens, have been proposed. To assess the reliability of some of these PCR tests in routine laboratory diagno...

A gene probe, CAP-21, which demonstrated interrelationships between the members of the Mycoplusma mycoides cluster w a s developed. The probe easily differentiated mycoplasmas in this cluster by clear and predictable hybridization patterns in Southern blots and separated the cluster into four groups. Strains of M. mycoides subsp, mycoides which were capable of causing contagious bovine pleuropn...

MCS4 RNA (125 nucleotides in length) is as abundant 5S rRNA in the cell and is encoded by two genes – mcs4a and mcs4b. We detected MCS4 RNA genes by random amplified polymorphic analysis (RAPD) and Southern hybridization technique. In our studies the genomic polymorphisms of Mycoplasma capricolum subsp. capricolum type strain California kid (ATCC 27343) and strain GM262G (ATCC 43092) were not d...

Several Mycoplasma species cause serious and economically important diseases in goats world-wide. Forty goat flocks in the Pishin district of Balochistan province of Pakistan were examined for the clinical cases of contagious caprine pleuropneumonia (CCPP) during 2008. Thirty goats suspected for CCPP on the basis of respiratory symptoms were euthanized for post mortem examination, microbiologic...

Mycoplasma capricolum subspecies capricolum (Mcc) is one of the causative agents of contagious agalactia (CA), which is an important disease in sheep and goats in the Mediterranean and Middle East countries. Mycoplasma agalactiae is the classic agent of CA in sheep and goats. Mycoplasma mycoides subspecies Capri (Mmc), Mycoplasma capricolum subspecies capricolum (Mcc), and Mycoplasma putrefacie...

Genome transplantation (GT) allows the installation of purified chromosomes into recipient cells, causing the resulting organisms to adopt the genotype and the phenotype conferred by the donor cells. This key process remains a bottleneck in synthetic biology, especially for genome engineering strategies of intractable and economically important microbial species. So far, this process has only b...