1. Lysine is readily incorporated into mycobactins P and S. Incorporation is into the hydroxamic acid moieties only and is equal in the mycobactic acid and cobactin portions of the molecule. 2. 2-Amino-6-hydroxyaminohexanoic acid is not taken up by cells of Mycobacterium phlei and is not detectable in extracts of cells actively synthesizing mycobactin. 3. The most abundant material derived from...

The Esx secretion pathway is conserved across Gram-positive bacteria. Esx-1, the best-characterized system, is required for virulence of Mycobacterium tuberculosis, although its precise function during infection remains unclear. Esx-3, a paralogous system present in all mycobacterial species, is required for growth in vitro. Here, we demonstrate that mycobacteria lacking Esx-3 are defective in ...

Recently some mycobactin-dependent mycobacteria were isolated from patients with Crohn's disease. These mycobacteria should be very similar to M. paratuberculosis which is closely related to M. avium. The author has investigated the relationship between M. avium, M. paratuberculosis and the mycobacteria associated with Crohn's disease.

The aim of this study was to use PARACHEK Johne's Absorbed EIA-CSL, gamma interferon test IFN BOVIGAM-CSL , Mycobacterial Growth Indicator Tube MGIT, BACTEC 960 BECTON-DICKINSON, PCR with IS900 probe, to diagnose paratuberculosis in sheep from the Argentine North East (ANE) and compare the results with those of the comparative skin testing using bovine and avian PPD (B-PPD and A-PPD), agar gel ...

Evidence is accumulating that Mycobacterium leprae is probably a microbe­ dependent microbe. l O1 3 The first report that multiplication of M. leprae was promoted by a growth factor of mycobacterial origin was proposed by Twort l 9 in 1 9 1 0 , two years before the discovery of the cultivation of 'lohne's bacilli' in media enriched with heat-killed mycobacteria . 2o The growth factor was identi...

Johne’s disease is characterized by chronic granulomatous enteritis of ruminants caused by Mycobacterium paratuberculosis infection. Early diagnosis of infected animals is necessary to reduce contamination of the environment and to control disease spread through feces (3, 6). About 75% of positive animals are either low or very low shedders (7, 8). Diagnostic samples for M. paratuberculosis ide...